Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice.
Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice.
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骨髓源性树突状细胞 (BMDC) 的过继转移可减轻 OVA 诱导的哮喘小鼠过敏性气道炎症。
DOI：
10.1038/s41598-020-70467-3
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发表时间：
2020
影响因子：
4.6
通讯作者：
Chen,Zhihong
中科院分区：
文献类型：
--
作者：
Xu,Kan;Wu,Nan;Min,Zhihui;Li,Zheng;Zhu,Tao;Liu,Chunfang;Zeng,Yuzhen;Song,Juan;Mao,Ruolin;Ji,Hong;Jiang,Zhilong;Chen,Zhihong
气道树突状细胞（DCs）被认为是过敏性炎症性疾病发病机制中的重要因素。细胞因子信号转导抑制因子3（SOCS 3）参与调节T细胞和巨噬细胞的功能，但表达SOCS 3的DC在变应性炎症性疾病中的作用仍存在争议。我们比较了过继转移SOCS 3-/-和SOCS 3 +/+骨髓来源的DC（BMDCs）对卵清蛋白（OVA）致敏哮喘小鼠气道炎症的影响。连续转移有或没有SOCS 3基因表达的成熟DC（脂多糖[LPS]诱导的DC，DClps）显著改善过敏性气道炎症。SOCS 3 −/−DCs轻微减弱BMDC诱导的免疫原性耐受。DCIps迁移到OVA致敏的肺中的效率高于未成熟的DC（DCim）。具有或不具有SOCS 3的DClp在过继转移到小鼠中后极大地改善了肺病理学评分并减轻了气道炎性细胞浸润;它们还增加了白细胞介素-10（IL-10）和转化生长因子-β（TGF-β）的产生，并抑制了OVA致敏后肺中的信号转导和转录激活因子（STAT）4和STAT 6信号传导。结论：BMDC过继转移诱导的免疫耐受可能不是由于SOCS 3基因缺失所致。BMDC过继转移可能成为通过调节免疫耐受和炎症之间的平衡来治疗哮喘的新途径。
Airway dendritic cells (DCs) are recognized as important factors in the mechanisms of allergic inflammatory diseases. Suppressor of cytokine signaling 3 (SOCS3) is involved in regulating the functions of T cells and macrophages, but the roles of SOCS3-expressing DCs in the pathogeneses of allergic inflammatory diseases are still controversial. We compared the effects of adoptively transferred SOCS3−/−and SOCS3+/+bone marrow-derived DCs (BMDCs) on airway inflammation in ovalbumin (OVA)-sensitized asthmatic mice. Adoptive transfer of mature DCs (lipopolysaccharide [LPS]-induced DCs, DClps) with or without SOCS3 gene expression significantly ameliorated allergic airway inflammation. SOCS3−/−DCs slightly attenuated BMDC-induced immunogenic tolerance. DClps migrated to OVA-sensitized lungs with higher efficiency than immature DCs (DCim). DClps with or without SOCS3 greatly improved lung pathology scores and alleviated airway inflammatory cell infiltration after adoptive transfer into mice; they also increased interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) production and inhibited signal transducer and activator of transcription (STAT) 4 and STAT6 signaling in the lungs after OVA sensitization. In conclusion, the BMDC adoptive transfer-induced immunogenic tolerance in OVA-sensitized mice might not be due to SOCS3 gene depletion. BMDC adoptive transfer may be developed into a new approach that alleviates asthma by modulating the balance between immune tolerance and inflammation.
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DOI：
--
发表时间：
2006
期刊：
Current Allergy and Asthma Reports 9
影响因子：
--
作者：
Kubo;M.;Inoue;H.
通讯作者：
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影响因子：
4.4
作者：
Nayyar, Aarti;Dawicki, Wojciech;Gordon, John R.
通讯作者：
Gordon, John R.
影响因子：
12.4
作者：
Huang, H.;Dawicki, W.;Gordon, J. R.
通讯作者：
Gordon, J. R.
影响因子：
4.4
作者：
Nakagome, K;Dohi, M;Yamamoto, K
通讯作者：
Yamamoto, K
影响因子：
4.3
作者：
Schneider, AM;Li, F;Gordon, JR
通讯作者：
Gordon, JR