Monitoring intra-cellular lipid metabolism in macrophages by Raman- and CARS-microscopy
Monitoring intra-cellular lipid metabolism in macrophages by Raman- and CARS-microscopy
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通过拉曼显微镜和 CARS 显微镜监测巨噬细胞的细胞内脂质代谢
DOI：
10.1117/12.854244
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发表时间：
2010
影响因子：
1.7
通讯作者：
J. Popp
中科院分区：
文献类型：
--
作者：
C. Matthäus;G. Bergner;C. Krafft;B. Dietzek;S. Lorkowski;J. Popp
单核细胞衍生的巨噬细胞在血管壁组织的脂质代谢中起关键作用。巨噬细胞可通过多种机制摄取脂质。作为吞噬细胞，巨噬细胞对于动脉壁内的脂质斑块的分解是重要的，所述脂质斑块有助于动脉硬化。特别感兴趣的是不同个体类型的脂质（例如脂肪酸、甘油三酯或游离和酯化胆固醇）的摄取动力学和细胞内命运。在这里，我们利用拉曼显微镜成像的脂质代谢，并遵循随后的存储或降解模式。光学显微镜与拉曼光谱学的组合允许在所采用的激光的衍射极限处可视化，并且通过相关的光谱信息进行生物化学表征。由于拉曼散射的弱点，相对长的测量时间可以通过非线性效应如相干反斯托克斯拉曼散射（汽车）来克服。有了这一贡献，我们介绍了第一个结果，以监测纳入个别细胞采用拉曼和汽车显微镜的脂质成分。
Monocyte-derived macrophages play a key role in lipid metabolism in vessel wall tissues. Macrophages can take up lipids by various mechanisms. As phagocytes, macrophages are important for the decomposition of lipid plaques within arterial walls that contribute to arteriosclerosis. Of special interest are uptake dynamics and intra-cellular fate of different individual types of lipids as, for example, fatty acids, triglycerides or free and esterified cholesterol. Here we utilize Raman microscopy to image the metabolism of such lipids and follow subsequent storage or degradation patterns. The combination of optical microscopy with Raman spectroscopy allows visualization at the diffraction limit of the employed laser light and biochemical characterization through the associated spectral information. Relatively long measuring times, due to the weakness of Raman scattering can be overcome by non-linear effects such as coherent anti-Stokes Raman scattering (CARS). With this contribution we introduce first results to monitor the incorporation of lipid components into individual cells employing Raman and CARS microscopy.
影响因子：
13.3
作者：
G. Bergner;Susana Chatzipapadopoulos;D. Akimov;B. Dietzek;D. Malsch;T. Henkel;S. Schlücker;J. Popp
通讯作者：
G. Bergner;Susana Chatzipapadopoulos;D. Akimov;B. Dietzek;D. Malsch;T. Henkel;S. Schlücker;J. Popp
影响因子：
4.9
作者：
Matthaeus, Christian;Kale, Amit;Diem, Max
通讯作者：
Diem, Max