Hemodynamic and neuropathological analysis in rats with aluminum trichloride-induced Alzheimer's disease.
Hemodynamic and neuropathological analysis in rats with aluminum trichloride-induced Alzheimer's disease.
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DOI：
10.1371/journal.pone.0082561
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hseu RS
中科院分区：
文献类型：
--
作者：
Chen SM;Fan CC;Chiue MS;Chou C;Chen JH;Hseu RS
血流动力学的正常对于保持脑血管的完整性至关重要，因此，保护阿尔茨海默病患者的认知功能。本研究旨在探讨三氯化铝诱导的阿尔茨海默病的血流动力学改变和神经病理变化的意义。实验动物为8~12周龄雄性Wistar大鼠。将大鼠随机分为2组：对照组和(+)对照组。在22周内每天记录食物摄入量、水摄入量和体重变化。同时，用磁共振成像(MRI)检测AlCl3诱导的AD模型大鼠的局部脑血流量(RCBF)。用电脑自动旋转流变仪分析血液流变学参数。用免疫组织化学染色分析受试者脑组织中的β-淀粉样蛋白(A-β)水平。血流动力学分析结果显示，(+)对照组的全血粘度(WBV)、纤维蛋白原、血浆粘度、红细胞聚集指数(RAI)显著高于对照组，而全血红细胞电泳值(EI)显著低于对照组。(+)对照组的乙酰胆碱酯酶-红细胞(AChE-RBC)结果明显高于对照组。结果还表明，AlCl3诱导的AD大鼠的rCBF下降约为正常大鼠的50%~60%。免疫组化染色结果显示，A-β斑块在(+)对照组大鼠的海马区和皮质积聚明显多于对照组。这一结果强调了血液流变学的重要性，并加强了血液动力学与神经病理变化之间的特异性联系。血液流变学参数，如WBV和纤维蛋白原，以及AChE-RBC最终被证明是反映AD患者严重程度和进展的有用的生物标志物。此外，在治疗干预中，这些参数可以替代有创性检查。
Hemodynamic normality is crucial to maintaining the integrity of cerebral vessels and, therefore, preserving the cognitive functions of Alzheimer's disease patients. This study investigates the implications of the hemodynamic changes and the neuropathological diversifications of AlCl3-induced AD. The experimental animals were 8- to 12-wk-old male Wistar rats. The rats were randomly divided into 2 groups: a control group and a (+)control group. Food intake, water intake, and weight changes were recorded daily for 22 wk. Synchronously, the regional cerebral blood flow (rCBF) of the rats with AlCl3-induced AD were measured using magnetic resonance imaging (MRI). The hemorheological parameters were analyzed using a computerized auto-rotational rheometer. The brain tissue of the subjects was analyzed using immunohistological chemical (IHC) staining to determine the beta-amyloid (Aβ) levels. The results of hemodynamic analysis revealed that the whole blood viscosity (WBV), fibrinogen, plasma viscosity and RBC aggregation index (RAI) in (+)control were significantly higher than that of control group, while erythrocyte electrophoresis (EI) of whole blood in (+)control were significantly lower than that of control group. The results of acetylcholinesterase-RBC (AChE-RBC)in the (+)control group was significantly higher than that of the control group. The results also show that the reduction of rCBF in rats with AlCl3-induced AD was approximately 50% to 60% that of normal rats. IHC stain results show that significantly more Aβ plaques accumulated in the hippocampus and cortex of the (+)control than in the control group. The results accentuate the importance of hemorheology and reinforce the specific association between hemodynamic and neuropathological changes in rats with AlCl3-induced AD. Hemorheological parameters, such as WBV and fibrinogen, and AChE-RBC were ultimately proven to be useful biomarkers of the severity and progression of AD patients. In addition, the parameters can be substituted for invasive inspection in therapeutic intervention.
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影响因子：
16.2
作者：
Cortes-Canteli, Marta;Paul, Justin;Norris, Erin H.;Bronstein, Robert;Ahn, Hyung Jin;Zamolodchikov, Daria;Bhuvanendran, Shivaprasad;Fenz, Katherine M.;Strickland, Sidney
通讯作者：
Strickland, Sidney
影响因子：
2.4
作者：
Chang, Chia-Yu;Liang, Hong-Jen;Liu, Der-Zen
通讯作者：
Liu, Der-Zen
影响因子：
4.8
作者：
Davis, J;Xu, F;Van Nostrand, WE
通讯作者：
Van Nostrand, WE
影响因子：
4.2
作者：
Campbell, A;Becaria, A;Bondy, SC
通讯作者：
Bondy, SC
影响因子：
3.8
作者：
Flaten, TP
通讯作者：
Flaten, TP