Genetic polymorphisms of the sortase A gene and social-behavioural factors associated with caries in children: a case-control study.
Genetic polymorphisms of the sortase A gene and social-behavioural factors associated with caries in children: a case-control study.
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DOI：
10.1186/s12903-015-0039-1
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发表时间：
2015-05-02
期刊：
影响因子：
2.9
通讯作者：
Lin HC
中科院分区：
文献类型：
--
作者：
Yu LX;Tao Y;Qiu RM;Zhou Y;Zhi QH;Lin HC
变形链球菌是引起龋病的主要病原菌。山梨酸酶是一种转肽酶，可以将几种表面蛋白锚定在变形链球菌的细胞壁上，并已被证明在致龋性中起着重要作用。本研究的目的是探讨变形链球菌感染儿童龋病相关的社会行为因素和排序酶基因(SrtA)的遗传多态性。在本病例对照研究中，分别从无龋组和高龋组儿童中选择了12 1株 变形链球菌进行srtA基因测序。社会和行为数据通过自我管理的问卷收集。从变形链球菌中提取基因组DNA，通过聚合酶链式反应扩增得到srtA基因。用ABI Variant Reporter软件对纯化的扩增产物进行测序和突变分析。比较两组间错义突变的分布和社会行为因素的平均值。采用多元Logistic回归模型对混杂因素进行控制。第168位(P = 0.023)和第470位(P = 0.032)突变频率在两组间差异有统计学意义。最佳拟合模型显示，年龄较大、高固体糖摄入量、母乳喂养时间长、可见菌斑比例高、SrtA基因第168位带有T的变形链球菌与儿童重度龋病相关(P <； 005)。与携带T的儿童相比，携带G168位点突变的儿童患重度龋病的危险性降低(OR = 为0.32，95%CI = 为0.12~0.86)。此外，年龄、母乳喂养时间、固体糖摄入量和糟糕的口腔卫生也是导致这种复杂疾病的原因之一。
Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. Sortase is a transpeptidase that anchors several surface proteins to the S. mutans cell wall and has been shown to play a major role in cariogenicity. The purpose of this study was to explore the genetic polymorphisms of the sortase gene (srtA) and the social-behavioural factors associated with dental caries in children with S. mutans. In this case–control study, 121 S. mutans strains were separately selected from caries-free children and high-severity caries children for sequencing of the srtA gene. Social and behavioural data were collected by self-administered questionnaires. Genomic DNA was extracted from S. mutans strains and amplified by PCR to obtain the srtA gene. The purified PCR products were sequenced and analysed for mutations with ABI Variant Reporter software. The distribution of missense mutations and the mean of social-behavioural factors were compared between the groups. A multiple logistic regression model was used to control for confounding factors. The mutation frequencies at loci 168 (P = 0.023) and 470 (P = 0.032) were significantly different between the groups. The best-fitting model showed that greater age, high frequencies of solid sugar consumption, prolonged breastfeeding, a high proportion of visible plaque, and S. mutans with a T at locus 168 of the srtA gene were associated with high-severity caries in children (P < 0.05). Children carrying a G at locus 168 of S. mutans had a decreased risk for high-severity caries (OR = 0.32, 95% CI = 0.12–0.86) compared with those carrying a T. The present study suggested that the locus 168 missense mutation of the srtA gene may correlate with caries susceptibility in children with S. mutans. In addition, age, duration of breastfeeding, solid sugar consumption, and poor oral hygiene contributed to this complex disease.
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影响因子：
2.9
作者：
Folayan, Morenike O.;Chukwumah, Nneka M.;Olatosi, Olubukola O.
通讯作者：
Olatosi, Olubukola O.
影响因子：
1.8
作者：
Mulu W;Demilie T;Yimer M;Meshesha K;Abera B
通讯作者：
Abera B
影响因子：
2.1
作者：
Zhou, Y.;Yang, J. Y.;Lin, H. C.
通讯作者：
Lin, H. C.
DOI：
10.1007/s10096-013-1993-7
发表时间：
2014-04
影响因子：
4.5
作者：
Krzysciak, W.;Jurczak, A.;Koscielniak, D.;Bystrowska, B.;Skalniak, A.
通讯作者：
Skalniak, A.
影响因子：
4.5
作者：
Moye ZD;Zeng L;Burne RA
通讯作者：
Burne RA