A preliminary survey of filarial parasites in dogs and cats in Sri Lanka.
A preliminary survey of filarial parasites in dogs and cats in Sri Lanka.
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DOI：
10.1371/journal.pone.0206633
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
de Silva NR
中科院分区：
文献类型：
--
作者：
Mallawarachchi CH;Chandrasena NTGA;Wickramasinghe S;Premaratna R;Gunawardane NYIS;Mallawarachchi NSMSM;de Silva NR
人类布鲁氏丝虫病在沉寂了40年后在斯里兰卡重新出现。本研究调查了犬和猫丝虫病在3个人类亚周期布鲁氏丝虫病地区的流行情况，以确定其潜在的宿主状态。在三个地点（Madampe、Wattala和Weliweriya）的布鲁氏丝虫病指示病例半径350米范围内，使用吉姆萨染色的厚血涂片对所有可接触到的流浪和家养狗和猫进行微丝虫病筛查。对3个地点的犬、猫微丝虫镜检阳性的代表性血液样本，采用核糖体DNA内转录间隔区2 （ITS2）特异引物进行PCR分析。共筛选了250只狗和134只猫。犬（68.8%）和猫（47.8%）的微丝虫病总体发生率均较高。狗的微丝虫病患病率显著高于猫（p<0.05）。根据微丝虫形态鉴定出2种丝虫，分别为犬（54.4%）和猫（34.3%）和布鲁贾（51.6%）种（犬，30.6%）。对犬（53例）和猫（24例）样本进行PCR分析，在615bp区域出现条带，证实感染了马来布鲁氏菌。在36份犬和17份猫的样本中，采用PCR检测到与D.(N.) repens共感染，在484bp处增加一条条带。Madampe市（农村）犬（81.8%）和猫（75%）总体微丝虫病率显著高于Wattala市(犬（62.4%）和猫（26.0%）（p<0.05）。人畜共患丝虫病的高感染率强烈暗示，在斯里兰卡，狗和猫是人类丝虫病和布鲁氏丝虫病的潜在宿主。
Human brugian filariasis has re-emerged in Sri Lanka after a quiescent period of four decades. This study investigated the prevalence of canine and feline filarial parasites in three localities with human sub-periodic brugian filariasis, in order to determine their potential reservoir status. All reachable dogs and cats, both stray and domestic, within a 350m radius of an index case of brugian filariasis in three locations (Madampe, Wattala and Weliweriya) were screened for microfilariae using Giemsa stained thick blood smears. A representative sample of canine and feline blood samples positive for Brugia spp. microfilariae by microscopy, from each of the three locations, were further analyzed by PCR with specific primers for internal transcribed spacer region 2 (ITS2) of the ribosomal DNA. A total of 250 dogs and 134 cats were screened. The overall microfilaraemia rates were high among both dogs (68.8%) and cats (47.8%). The prevalence of microfilaraemia was significantly higher among dogs than cats (p<0.05). Two filarial species were identified based on morphology of microfilariae: Dirofilaria (Nochtiella) repens (dogs, 54.4% and cats, 34.3%) and Brugia spp. (dogs, 51.6% and cats, 30.6%). PCR analysis of canine (n = 53) and feline (n = 24) samples elicited bands in the region of 615bp, which confirmed Brugia malayi infection. Co-infection with D.(N.) repens was detected by PCR with an additional band at 484bp, in 36 canine and 17 feline samples. Overall microfilaraemia rates of dogs (81.8%) and cats (75%) in Madampe (rural) were significantly higher than in urbanized Wattala (dogs, 62.4% and cats, 26.0%) (p<0.05). High rates of zoonotic filarial infections strongly implicate dogs and cats as potential reservoirs for human dirofilariasis and brugian filariasis in Sri Lanka.
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影响因子：
2.6
作者：
Casiraghi, Maurizio;Bazzocchi, Chiara;Genchi, Claudio
通讯作者：
Genchi, Claudio
影响因子：
2.6
作者：
Rishniw, M;Barr, SC;Alpizar, JLD
通讯作者：
Alpizar, JLD
影响因子：
1.6
作者：
PALMIERI, JR;MASBAR, S;DARWIS, F
通讯作者：
DARWIS, F
DOI：
10.1007/s12639-013-0348-8
发表时间：
2015-06-01
期刊：
Journal of parasitic diseases : official organ of the Indian Society for Parasitology
影响因子：
--
作者：
Kini, Reshma G;Leena, J B;George, Lovely
通讯作者：
George, Lovely
影响因子：
4
作者：
Irwin, PJ
通讯作者：
Irwin, PJ