Correlation between a quantitative anti-SARS-CoV-2 IgG ELISA and neutralization activity.
Correlation between a quantitative anti-SARS-CoV-2 IgG ELISA and neutralization activity.
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DOI：
10.1002/jmv.27287
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发表时间：
2022-01
影响因子：
12.7
通讯作者：
Hartmann G
中科院分区：
文献类型：
--
作者：
Dolscheid-Pommerich R;Bartok E;Renn M;Kümmerer BM;Schulte B;Schmithausen RM;Stoffel-Wagner B;Streeck H;Saschenbrecker S;Steinhagen K;Hartmann G
在当前的 COVID-19 大流行中，有必要更好地了解仅结合抗体和功能中和抗体之间的关系，以表征感染或疫苗接种后的保护性抗病毒免疫。本研究分析了新型定量 EUROIMMUN 抗 SARS-CoV-2 QuantiVac ELISA (IgG) 与微量中和测定之间的相关性水平。使用来自 COVID-19 爆发研究人群的 123 份血浆样本（通过半定量抗 SARS-CoV-2 IgG 测试预选）来评估新型定量 ELISA (IgG) 和微量中和测定之间的关系。使用 QuantiVac ELISA 在 106 个 (86.2%) 样本中检测到针对 S1 抗原的结合 IgG，而 89 个 (72.4%) 样本显示出中和抗体活性。 Spearman 的相关分析表明，抗 S1 IgG 水平与中和抗体滴度之间存在很强的正相关关系 (rs = 0.819，p < 0.0001)。高和低抗 S1 IgG 水平分别与高滴度中和抗体的阳性预测值 72.0% 和低滴度中和抗体的阴性预测值 90.8% 相关。这些结果证实了 QuantiVac ELISA 的实施，以评估感染或疫苗接种后的保护性免疫力。将一种新的定量抗 S1 测定（EUROIMMUN 抗 SARS-CoV-2 QuantiVac ELISA IgG）与微量中和测定进行了比较。 QuantiVac ELISA 显示出高水平的定性总体一致性以及与中和测试结果的强定量相关性。 QuantiVac ELISA 有潜力预测感染或疫苗接种后针对 SARS-CoV-2 的保护性免疫力。
In the current COVID‐19 pandemic, a better understanding of the relationship between merely binding and functionally neutralizing antibodies is necessary to characterize protective antiviral immunity following infection or vaccination. This study analyzes the level of correlation between the novel quantitative EUROIMMUN Anti‐SARS‐CoV‐2 QuantiVac ELISA (IgG) and a microneutralization assay. A panel of 123 plasma samples from a COVID‐19 outbreak study population, preselected by semiquantitative anti‐SARS‐CoV‐2 IgG testing, was used to assess the relationship between the novel quantitative ELISA (IgG) and a microneutralization assay. Binding IgG targeting the S1 antigen was detected in 106 (86.2%) samples using the QuantiVac ELISA, while 89 (72.4%) samples showed neutralizing antibody activity. Spearman's correlation analysis demonstrated a strong positive relationship between anti‐S1 IgG levels and neutralizing antibody titers (r s = 0.819, p < 0.0001). High and low anti‐S1 IgG levels were associated with a positive predictive value of 72.0% for high‐titer neutralizing antibodies and a negative predictive value of 90.8% for low‐titer neutralizing antibodies, respectively. These results substantiate the implementation of the QuantiVac ELISA to assess protective immunity following infection or vaccination. A new quantitative anti‐S1 assay (EUROIMMUN Anti‐SARS‐CoV‐2 QuantiVac ELISA IgG) was compared to a microneutralization assay. The QuantiVac ELISA showed a high level of qualitative overall agreement and a strong quantitative correlation with the results of neutralization testing. The QuantiVac ELISA has the potential to predict protective immunity against SARS‐CoV‐2 folllowing infection or vaccination.
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DOI：
10.1093/infdis/jiaa784
发表时间：
2021-03-29
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Klingler J;Weiss S;Itri V;Liu X;Oguntuyo KY;Stevens C;Ikegame S;Hung CT;Enyindah-Asonye G;Amanat F;Baine I;Arinsburg S;Bandres JC;Kojic EM;Stoever J;Jurczyszak D;Bermudez-Gonzalez M;Nádas A;Liu S;Lee B;Zolla-Pazner S;Hioe CE
通讯作者：
Hioe CE
DOI：
10.1111/tme.12746
发表时间：
2021-06
期刊：
Transfusion medicine (Oxford, England)
影响因子：
--
作者：
Harvala H;Robb ML;Watkins N;Ijaz S;Dicks S;Patel M;Supasa P;Wanwisa D;Liu C;Mongkolsapaya J;Bown A;Bailey D;Vipond R;Grayson N;Temperton N;Gupta S;Ploeg RJ;Bolton J;Fyfe A;Gopal R;Simmonds P;Screaton G;Thompson C;Brooks T;Zambon M;Miflin G;Roberts DJ
通讯作者：
Roberts DJ
影响因子：
6.4
作者：
Jahrsdoerfer, Bernd;Kroschel, Joris;Schrezenmeier, Hubert
通讯作者：
Schrezenmeier, Hubert
影响因子：
64.8
作者：
Zost SJ;Gilchuk P;Case JB;Binshtein E;Chen RE;Nkolola JP;Schäfer A;Reidy JX;Trivette A;Nargi RS;Sutton RE;Suryadevara N;Martinez DR;Williamson LE;Chen EC;Jones T;Day S;Myers L;Hassan AO;Kafai NM;Winkler ES;Fox JM;Shrihari S;Mueller BK;Meiler J;Chandrashekar A;Mercado NB;Steinhardt JJ;Ren K;Loo YM;Kallewaard NL;McCune BT;Keeler SP;Holtzman MJ;Barouch DH;Gralinski LE;Baric RS;Thackray LB;Diamond MS;Carnahan RH;Crowe JE Jr
通讯作者：
Crowe JE Jr
影响因子：
8.8
作者：
Gasser R;Cloutier M;Prévost J;Fink C;Ducas É;Ding S;Dussault N;Landry P;Tremblay T;Laforce-Lavoie A;Lewin A;Beaudoin-Bussières G;Laumaea A;Medjahed H;Larochelle C;Richard J;Dekaban GA;Dikeakos JD;Bazin R;Finzi A
通讯作者：
Finzi A