Detailed histological structure of human hair follicle bulge region at different ages: A visible niche for nesting adult stem cells
Detailed histological structure of human hair follicle bulge region at different ages: A visible niche for nesting adult stem cells
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不同年龄的人类毛囊隆起区域的详细组织学结构：筑巢成体干细胞的可见生态位
DOI：
10.1007/s11596-012-1012-8
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发表时间：
2012
影响因子：
--
通讯作者：
Lei, Tiechi
中科院分区：
文献类型：
--
作者：
Wang, Xiong;Shi, Ying;Zhou, Qiong;Liu, Xiaoming;Xu, Shizheng;Lei, Tiechi
在毛囊的隆起区，立毛肌(APM)包围着一个密集而向中心排列的细胞团，APM为容纳异种成体干细胞提供了专门化的微环境(NICE)。然而，隆起区的详细组织结构和细胞组成值得深入研究，并可能对毛囊生长调节有影响。这项研究旨在确定根据可检测到的自体荧光的存在而制备的拔毛毛囊中部的假定干细胞和谱系特异性前体细胞的基因表达谱。该结构还通过使用连续切片技术来表征。采用显微解剖技术，精确切除毛囊内具有自发荧光的隆起区域。采用半定量逆转录聚合酶链式反应(RT-PCR)检测毛囊隆起区上皮细胞、黑素细胞和基质干细胞的基因表达谱。用连续切片和免疫组织化学染色方法，对9例30周~75岁患者的头皮皮肤标本的毛囊自体荧光段隆起区的形态及其随年龄的变化进行了研究。基因表达谱分析显示，在毛囊的隆起区有DctHiTyraseLo-Tyrp1LoMC1RLoMITFLo/K15Hi/NPNTHiin基因转录的细胞，并带有自发荧光片段，这与毛球中的模式不同。免疫组织化学染色显示，在毛囊连续切片中，APM植入水平可见从外根鞘(ORS)萌发出的小细胞突起，其中充满了K15+/K19+表达细胞。同样，平滑肌肌动蛋白阳性的APM肌束紧密地包围着这些细胞突起，胎儿头皮皮肤细胞突起的出现频率高于成人头皮皮肤。这项研究提供的证据表明，相对于APM插入，ORS处发生的细胞突起更有可能是充斥着大量干细胞的可见壁龛的特征。这些细胞突起在胎儿头皮组织中的出现频率(128%)显著高于年轻人(49.4%)和老年人(25.4%)(P<；0.01)，但在两个成年组之间差异不显著。这些结果表明，这些细胞突起为无数干细胞和/或前体细胞提供了一个利基场所，以满足胚胎毛囊发育的需要。本研究所采用的显微解剖方法简单、可靠，可利用毛囊的自体荧光片段切除毛囊的隆起区，对干细胞培养的研究具有重要价值。
In the bulge region of the hair follicle, a densely and concentrically packed cell mass is encircled by the arrector pili muscle (APM), which offers a specilized microenvironment (niche) for housing heterogeneous adult stem cells. However, the detailed histological architecture and the cellular composition of the bulge region warrants intensive study and may have implications for the regulation of hair follicle growth regulation. This study was designed to define the gene-expression profiles of putative stem cells and lineage-specific precursors in the mid-portions of plucked hair follicles prepared according to the presence of detectable autofluorescence. The structure was also characterized by using a consecutive sectioning technique. The bulge region of the hair follicle with autofluorescence was precisely excised by employing a micro-dissection procedure. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was performed to identify the gene expression profiles specific for epithelial, melanocyte and stromal stem cells in the bulge region of the hair follicle visualized by autofluorescence. The morphology and its age-dependent changes of bulge region of the hair follicles with autofluorescence segment were also examined in 9 scalp skin specimens collected from patients aged 30 weeks to 75 years, by serial sectioning and immuno-staining. Gene expression profile analysis revealed that there were cells with mRNA transcripts of DctHiTyraseLo-Tyrp1LoMC1RLoMITFLo/K15Hi/NPNTHiin the bulge region of the hair follicle with autofluorescence segments, which differed from the patterns in hair bulbs. Small cell-protrusions that sprouted from the outer root sheath (ORS) were clearly observed at the APM inserting level in serial sections of hair follicles by immunohistological staining, which were characteristically replete with K15+/K19+expressing cells. Likewise, the muscle bundles of APM positive for smooth muscle actin intimately encircled these cell-protrusions, and the occurrence frequency of the cell-protrusions was increased in fetal scalp skin compared with adult scalp skin. This study provided the evidence that the cell-protrusions occurring at the ORS relative to the APM insertion are more likely to be characteristic of the visible niches that are filled with abundant stem cells. The occurrence frequency of these cell-protrusions was significantly increased in fetal scalp skin samples (128%) as compared with the scalp skins of younger (49.4%) and older (25.4%) adults (P<0.01), but difference in the frequency between the two adult groups were not significant. These results indicated that these cell-protrusions function as a niche house for the myriad stem cells and/or precursors to meet the needs of the development of hair follicles in an embryo. The micro-dissection used in this study was simple and reliable in excising the bulge region of the hair follicle with autofluorescence segments dependent on their autofluorescence is of value for the study of stem cell culture.
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影响因子：
64.5
作者：
Rabbani P;Takeo M;Chou W;Myung P;Bosenberg M;Chin L;Taketo MM;Ito M
通讯作者：
Ito M
影响因子：
64.5
作者：
Fujiwara H;Ferreira M;Donati G;Marciano DK;Linton JM;Sato Y;Hartner A;Sekiguchi K;Reichardt LF;Watt FM
通讯作者：
Watt FM
影响因子：
2.4
作者：
Song, WC;Hwang, WJ;Koh, KS
通讯作者：
Koh, KS
DOI：
10.1111/j.0022-202x.2005.23649.x
发表时间：
2005-04
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
B. Wun;Qingfeng Tao;S. Lyle
通讯作者：
B. Wun;Qingfeng Tao;S. Lyle
影响因子：
4.7
作者：
Jie Zhao;Liangqi Liu;Ying-Juan Wang;Wei Yang;Wen-Xin Geng;Jing Wei;Li-Wen Li-Li-Wen-Li-8447555;Fu-lin Chen
通讯作者：
Jie Zhao;Liangqi Liu;Ying-Juan Wang;Wei Yang;Wen-Xin Geng;Jing Wei;Li-Wen Li-Li-Wen-Li-8447555;Fu-lin Chen