Probing the (H3-H4)2 histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling.
Probing the (H3-H4)2 histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling.
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使用脉冲EPR光谱与位置定向的自旋标记探测（H3-H4）2组蛋白四聚体结构。
DOI：
10.1093/nar/gkp1003
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发表时间：
2010-01
影响因子：
14.9
通讯作者：
Norman DG
中科院分区：
文献类型：
--
作者：
Bowman A;Ward R;El-Mkami H;Owen-Hughes T;Norman DG
（H3-H4）2组蛋白四聚体形成核小体的中心核心，因此在核小体的组装、拆卸和定位中起着重要作用。尽管它在染色质中的基本作用，四聚体已收到很少的结构研究。在这里，通过使用脉冲电子-电子双共振光谱加上定点自旋标记，我们调查的四聚体在溶液中的结构。我们发现，四聚体是结构上更异质性的自己比隔离在八聚体或核小体。特别是，虽然包括H3-H3′界面的中心区域保留了与核小体中观察到的结构相似的结构，但其他区域如H3 αN螺旋显示出增加的结构异质性。游离四聚体中H3 αN螺旋的灵活性也说明了翻译后修饰改变该区域结构并介导与组蛋白伴侣相互作用的潜力。这里描述的方法有望证明一个强大的系统，用于研究组蛋白与染色质组装/流动性中的其他重要因素的额外组装的结构。
The (H3-H4)2 histone tetramer forms the central core of nucleosomes and, as such, plays a prominent role in assembly, disassembly and positioning of nucleosomes. Despite its fundamental role in chromatin, the tetramer has received little structural investigation. Here, through the use of pulsed electron-electron double resonance spectroscopy coupled with site-directed spin labelling, we survey the structure of the tetramer in solution. We find that tetramer is structurally more heterogeneous on its own than when sequestered in the octamer or nucleosome. In particular, while the central region including the H3-H3′ interface retains a structure similar to that observed in nucleosomes, other regions such as the H3 αN helix display increased structural heterogeneity. Flexibility of the H3 αN helix in the free tetramer also illustrates the potential for post-translational modifications to alter the structure of this region and mediate interactions with histone chaperones. The approach described here promises to prove a powerful system for investigating the structure of additional assemblies of histones with other important factors in chromatin assembly/fluidity.
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影响因子：
16
作者：
Badis, Gwenael;Chan, Esther T.;van Bakel, Harm;Pena-Castillo, Lourdes;Tillo, Desiree;Tsui, Kyle;Carlson, Clayton D.;Gossett, Andrea J.;Hasinoff, Michael J.;Warren, Christopher L.;Gebbia, Marinella;Talukder, Shaheynoor;Yang, Ally;Mnaimneh, Sanie;Terterov, Dimitri;Coburn, David;Yeo, Ai Li;Yeo, Zhen Xuan;Clarke, Neil D.;Lieb, Jason D.;Ansari, Aseem Z.;Nislow, Corey;Hughes, Timothy R.
通讯作者：
Hughes, Timothy R.
影响因子：
16.8
作者：
通讯作者：
--
影响因子：
9.8
作者：
Dalal Y;Wang H;Lindsay S;Henikoff S
通讯作者：
Henikoff S
影响因子：
14.9
作者：
Cai Q;Kusnetzow AK;Hubbell WL;Haworth IS;Gacho GP;Van Eps N;Hideg K;Chambers EJ;Qin PZ
通讯作者：
Qin PZ
影响因子：
5.3
作者：
Fillingham, Jeffrey;Recht, Judith;Greenblatt, Jack F.
通讯作者：
Greenblatt, Jack F.