Modular enzymatically crosslinked protein polymer hydrogels for in situ gelation.
Modular enzymatically crosslinked protein polymer hydrogels for in situ gelation.
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DOI：
10.1016/j.biomaterials.2010.06.003
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发表时间：
2010-10
期刊：
影响因子：
14
通讯作者：
Barron, Annelise E.
中科院分区：
文献类型：
--
作者：
Davis, Nicolynn E.;Ding, Sheng;Forster, Ryan E.;Pinkas, Daniel M.;Barron, Annelise E.
在模块化和交联化学方面模仿细胞外基质的生物材料有可能概括最终控制细胞命运的指导性信号。为了实现这一目标，模块化蛋白质聚合物水凝胶通过基因工程和酶交联被创造出来。利用动物源性组织转谷氨酰胺酶（tTG）和重组人转谷氨酰胺酶（hTG）酶偶联两类含有赖氨酸或谷氨酰胺的蛋白质聚合物，它们具有沿蛋白质主链均匀分布的酶交联识别底物。在生理条件下使用tTG，通过颗粒跟踪微流变学确定，交联发生在两分钟内。水凝胶组成对凝胶的弹性储存模量的影响超过4倍，对凝胶的微观结构和溶胀程度也有影响，但对纤溶酶的降解没有明显影响。小鼠3T3和人原代成纤维细胞均在二维和三维培养，细胞活力未下降，并在二维中呈现扩散。这些凝胶的性质是通过蛋白质聚合物前体的特定性质控制的，使这些凝胶对原位治疗有价值。此外，模块化的水凝胶组合物允许为特定的组织工程应用定制机械和物理特性。
Biomaterials that mimic the extracellular matrix in both modularity and crosslinking chemistry have the potential to recapitulate the instructive signals that ultimately control cell fate. Toward this goal, modular protein polymer-based hydrogels were created through genetic engineering and enzymatic crosslinking. Animal derived tissue transglutaminase (tTG) and recombinant human transglutaminase (hTG) enzymes were used for coupling two classes of protein polymers containing either lysine or glutamine, which have the recognition substrates for enzymatic crosslinking, evenly spaced along the protein backbone. Utilizing tTG under physiological conditions, crosslinking occurred within two minutes, as determined by particle tracking microrheology. Hydrogel composition impacted the elastic storage modulus of the gel over 4-fold and also influenced microstructure and degree of swelling, but did not appreciably effect degradation by plasmin. Mouse 3T3 and primary human fibroblasts were cultured in both 2- and 3-dimensions without a decrease in cell viability and displayed spreading in 2D. The properties of these gels, which are controlled through the specific nature of the protein polymer precursors, render these gels valuable for in situ therapies. Furthermore, the modular hydrogel composition allows tailoring of mechanical and physical properties for specific tissue engineering applications.
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影响因子：
14
作者：
Ehrbar, Martin;Rizzi, Simone C.;Lutolf, Matthias P.
通讯作者：
Lutolf, Matthias P.
影响因子：
4.1
作者：
Nettles, Dana L.;Kitaoka, Kenichi;Setton, Lori A.
通讯作者：
Setton, Lori A.
影响因子：
8.6
作者：
MASON, TG;WEITZ, DA
通讯作者：
WEITZ, DA
影响因子：
6.2
作者：
Davis, Nicolynn E.;Karfeld-Sulzer, Lindsay S.;Ding, Sheng;Barron, Annelise E.
通讯作者：
Barron, Annelise E.
影响因子：
6.2
作者：
Halstenberg, S;Panitch, A;Hubbell, JA
通讯作者：
Hubbell, JA