Synthesis and characterization of a new class of cationic protein polymers for multivalent display and biomaterial applications.
Synthesis and characterization of a new class of cationic protein polymers for multivalent display and biomaterial applications.
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DOI：
10.1021/bm801348g
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发表时间：
2009-05-11
影响因子：
6.2
通讯作者：
Barron, Annelise E.
中科院分区：
文献类型：
--
作者：
Davis, Nicolynn E.;Karfeld-Sulzer, Lindsay S.;Ding, Sheng;Barron, Annelise E.
通过生物合成技术工程化的单分散蛋白质聚合物非常适合用作用于生物材料应用的梳状聚合物结构的基础。我们已经开发了一类新的线性，阳离子，无规卷曲蛋白质聚合物设计作为多价展示的支架。这些聚合物含有均匀间隔的赖氨酸残基，其允许侧基官能团的化学或酶促缀合。圆二色性（CD）光谱和浊度实验已经证实，这些蛋白质具有无规卷曲结构，并且在高达至少65 °C下可溶。细胞活力测定表明，这些构建体在浓度高达100 μM的溶液中无毒。我们已经成功地附加了一个小的生物活性肽，类肽-肽杂合物，聚（乙二醇）（PEG）聚合物，和荧光团的蛋白质聚合物通过化学或酶促偶联，证明其适合作为多价支架在溶液中或作为凝胶。
Monodisperse protein polymers engineered by biosynthetic techniques are well suited to serve as a basis for creating comb-like polymer architectures for biomaterial applications. We have developed a new class of linear, cationic, random-coil protein polymers designed to act as scaffolds for multivalent display. These polymers contain evenly spaced lysine residues that allow for chemical or enzymatic conjugation of pendant functional groups. Circular dichroism (CD) spectroscopy and turbidity experiments have confirmed that these proteins have a random coil structure and are soluble up to at least 65 °C. Cell viability assays suggest these constructs are non-toxic in solution up to a concentration of 100 μM. We have successfully attached a small bioactive peptide, a peptoid-peptide hybrid, a poly(ethylene glycol) (PEG) polymer, and a fluorophore to the protein polymers by chemical or enzymatic coupling, demonstrating their suitability to serve as multivalent scaffolds in solutions or as gels.
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