MALDI-ToF protein profiling as a potential rapid diagnostic platform for COVID-19.
MALDI-ToF protein profiling as a potential rapid diagnostic platform for COVID-19.
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DOI：
10.1016/j.jmsacl.2021.09.001
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发表时间：
2021-08
影响因子：
2.2
通讯作者：
Gaillard ER
中科院分区：
文献类型：
--
作者：
Chivte P;LaCasse Z;Seethi VDR;Bharti P;Bland J;Kadkol SS;Gaillard ER
在较宽的m/z范围内，比较新冠肺炎RT-qPCR和MALDI-ToF分析的状态。在选定的生物标志物范围内，≥与RT-qPCR90%符合新冠肺炎的状态。宿主和病毒蛋白可能存在于每个光谱中，这可能会提高诊断能力。在宣布新冠肺炎大流行一年多之后，仍然需要对当前和新出现的SARS-CoV-2毒株产生高度特异性和敏感性的准确、快速、廉价和非侵入性诊断方法。与鼻咽拭子相比，几项研究已经确定唾液是更容易检测SARS-CoV-2的样本类型。考虑到新冠肺炎检测的局限性和高要求，我们采用MALDI-TOF质谱仪对60份献血员含漱液样品进行了分析，并将分析结果与新冠肺炎的状况进行了比较。同时分析了几种标准，包括分离的人血清免疫球蛋白和对照，如新冠肺炎前唾液和热灭活的SARS-CoV-2病毒，以提供唾液和病毒蛋白质组在此工作流程中出现的相对视图。建立了5个与新冠肺炎阳性个体高度一致的潜在生物标志物峰。总体而言，这些结果与鼻咽拭子RT-qPCR检测的一致性在研究队列中的≥为90%，这些队列由年轻的和基本上没有症状的学生运动员组成。从临床角度来看，这项先导性研究的结果表明，MALDI-ToF可以用于开发一种相对快速和廉价的新冠肺炎检测方法。
Comparison of COVID-19 RT-qPCR status with MALDI-ToF analysis over a wide m/z range. ≥90% agreement with RT-qPCR COVID-19 status in the selected biomarker ranges. Host and viral proteins potentially present in each spectrum may enhance diagnostics. More than a year after the COVID-19 pandemic was declared, the need still exists for accurate, rapid, inexpensive and non-invasive diagnostic methods that yield high specificity and sensitivity towards the current and newly emerging SARS-CoV-2 strains. Compared to the nasopharyngeal swabs, several studies have established saliva as a more amenable specimen type for early detection of SARS-CoV-2. Considering the limitations and high demand for COVID-19 testing, we employed MALDI-ToF mass spectrometry in the analysis of 60 gargle samples from human donors and compared the resultant spectra against COVID-19 status. Several standards, including isolated human serum immunoglobulins, and controls, such as pre-COVID-19 saliva and heat inactivated SARS-CoV-2 virus, were simultaneously analyzed to provide a relative view of the saliva and viral proteome as they would appear in this workflow. Five potential biomarker peaks were established that demonstrated high concordance with COVID-19 positive individuals. Overall, the agreement of these results with RT-qPCR testing on nasopharyngeal swabs was ≥90% for the studied cohort, which consisted of young and largely asymptomatic student athletes. From a clinical standpoint, the results from this pilot study suggest that MALDI-ToF could be used to develop a relatively rapid and inexpensive COVID-19 assay.
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影响因子：
4.1
作者：
Herrera NG;Morano NC;Celikgil A;Georgiev GI;Malonis RJ;Lee JH;Tong K;Vergnolle O;Massimi AB;Yen LY;Noble AJ;Kopylov M;Bonanno JB;Garrett-Thomson SC;Hayes DB;Bortz RH 3rd;Wirchnianski AS;Florez C;Laudermilch E;Haslwanter D;Fels JM;Dieterle ME;Jangra RK;Barnhill J;Mengotto A;Kimmel D;Daily JP;Pirofski LA;Chandran K;Brenowitz M;Garforth SJ;Eng ET;Lai JR;Almo SC
通讯作者：
Almo SC
DOI：
10.3390/diagnostics10100746
发表时间：
2020-09-24
期刊：
Diagnostics (Basel, Switzerland)
影响因子：
--
作者：
Iles RK;Zmuidinaite R;Iles JK;Carnell G;Sampson A;Heeney JL
通讯作者：
Heeney JL
影响因子：
3.1
作者：
Rocca MF;Zintgraff JC;Dattero ME;Santos LS;Ledesma M;Vay C;Prieto M;Benedetti E;Avaro M;Russo M;Nachtigall FM;Baumeister E
通讯作者：
Baumeister E
影响因子：
4.4
作者：
Ihling, Christian;Taenzler, Dirk;Sinz, Andrea
通讯作者：
Sinz, Andrea
影响因子：
2.9
作者：
BANK, RA;HETTEMA, EH;PRONK, JC
通讯作者：
PRONK, JC