Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone.
Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone.
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草莓脉带病毒中国分离株全核苷酸序列及其全长DNA克隆的感染性
DOI：
10.1186/s12985-016-0624-1
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发表时间：
2016-10-06
期刊：
影响因子：
4.8
通讯作者：
Jiang T
中科院分区：
文献类型：
--
作者：
Feng M;Zhang H;Pan Y;Hu Y;Chen J;Zuo D;Jiang T
草莓脉带病毒（Strawberry vein banding virus，SVBV）是一种双链DNA植物病毒，已在北美、澳大利亚、巴西、日本、欧洲和我国多个省份发现。受感染的草莓植株表现出轻微的脉带症状和沿脉沿着褪绿。它是亚洲、欧洲和北美草莓种植区最重要的经济病害之一。从草莓（Fragaria × ananassa cv.在辽宁省沈阳市发现的Sachinoka）样本。序列分析表明，SVBV-CN与美国SVBV（SVBV-US）的同源性最高，为85.8%。基于花椰菜花叶病毒全基因组序列的系统发育分析，确定了两个主要分支。SVBV-CN与SVBV-US聚在一起，而其他花椰菜花叶病毒形成单独的分支。用SVBV-CN的感染性克隆接种野草莓，引起系统感染，沿主叶脉沿着出现明显的黄化带症状。这表明SVBV-CN感染性克隆可以重现在自然感染的草莓中观察到的症状，因此可能是在草莓中观察到的原始疾病的病原体。此外，使用真空渗透用感染性克隆接种的草莓植物在接种后4-5周内出现了具有86- 100%的非常高的感染率的症状。相比之下，接种后8-9周，使用双接种的感染率为20- 40%。从自然感染的草莓中测定了SVBV的全核苷酸序列。用SVBV-CN的侵染性克隆进行农杆菌渗入草莓植株，导致在中国辽宁省沈阳市的感染草莓中发现的典型症状。这是第一份描述SVBV-CN感染性克隆的报告，真空渗透可以作为一种新的和高效的手段接种草莓植株。本文的在线版本（doi：10.1186/s12985-016-0624-1）包含补充材料，可供授权用户使用。
Strawberry vein banding virus (SVBV) is a double-stranded DNA plant virus, which has been found in North America, Australia, Brazil, Japan, Europe and several provinces of China. Infected strawberry plants exhibit mild vein-banding symptoms and chlorosis along the veins. It is one of the most economically important diseases in Asiatic, European and North American strawberry-growing areas. The complete genome of an SVBV Chinese isolate (SVBV-CN) was isolated and cloned from a naturally infected strawberry (Fragaria × ananassa cv. Sachinoka) sample found in Shenyang city of Liaoning province. Sequence analysis revealed a complete genome of 7864 nucleotides (nts) that indicated SVBV-CN was most closely related to SVBV from the United States (SVBV-US) with a sequence similarity of 85.8 %. Two major clades were identified based on phylogenetic analysis of the complete genome sequences of caulimoviruses. SVBV-CN clustered together with SVBV-US, whereas other caulimoviruses formed a separate branch. Agrobacterium-mediated inoculation of Fragaria vesca with an infectious clone of SVBV-CN results in systemic infection with distinct symptoms of yellowing bands along the main leaf veins. This suggests that the SVBV-CN infectious clone can recapitulate the symptoms observed in naturally infected strawberries, and therefore is likely the causal agent of the original disease observed in strawberries. Furthermore, strawberry plants inoculated with the infectious clone using vacuum infiltration developed symptoms with a very high infection rate of 86–100 % in 4-5 weeks post-inoculation. This compares to an infection rate of 20–40 % in 8–9 weeks post-inoculation using syringe-inoculation. The complete nucleotide sequence of SVBV from a naturally infected strawberry was determined. Agroinfiltration of strawberry plants using an infectious clone of SVBV-CN resulted in symptoms typically found in infected strawberries from Shenyang city of Liaoning province in China. This is the first report describing an infectious clone of SVBV-CN, and that vacuum infiltration can be potentially used as a new and highly efficient means for inoculation of strawberry plants. The online version of this article (doi:10.1186/s12985-016-0624-1) contains supplementary material, which is available to authorized users.
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3.5
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