Dynamic changes of CD44 expression from progenitors to subpopulations of astrocytes and neurons in developing cerebellum.
Dynamic changes of CD44 expression from progenitors to subpopulations of astrocytes and neurons in developing cerebellum.
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DOI：
10.1371/journal.pone.0053109
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ishizaki Y
中科院分区：
文献类型：
--
作者：
Naruse M;Shibasaki K;Yokoyama S;Kurachi M;Ishizaki Y
我们之前报道过，CD44阳性细胞是发育中小脑中星形胶质细胞前体细胞的候选细胞，因为通过荧光激活细胞分选（FACS）选择的表达高水平CD44的细胞在体外仅产生星形胶质细胞。然而，CD44是否是体内小脑星形胶质细胞前体细胞的特异性细胞标志物尚不清楚。在本研究中，我们使用免疫组织化学、原位杂交和FACS来分析CD44的空间和时间表达，并表征发育过程中小鼠小脑中CD44阳性细胞的特征。 CD44 表达不仅在星形胶质细胞前体细胞中观察到，而且在出生后早期的神经干细胞和少突胶质细胞前体细胞 (OPC) 中也观察到。 OPCs 中的 CD44 表达在少突胶质细胞分化过程中被关闭。有趣的是，在发育过程中，CD44的表达仅限于小脑三种类型星形胶质细胞中的伯格曼胶质细胞和纤维性星形胶质细胞，并且星形胶质细胞中的表达在出生后发育过程中被关闭。 CD44 表达也在发育中的浦肯野神经元和颗粒神经元中检测到，但仅限于成人小脑中的颗粒神经元。因此，在小脑发育的早期阶段，CD44在几种类型的前体细胞中广泛表达，并且在发育过程中，CD44的表达变得仅限于成人的颗粒神经元。
We previously reported that CD44-positive cells were candidates for astrocyte precursor cells in the developing cerebellum, because cells expressing high levels of CD44 selected by fluorescence-activated cell sorting (FACS) gave rise only to astrocytes in vitro. However, whether CD44 is a specific cell marker for cerebellar astrocyte precursor cells in vivo is unknown. In this study, we used immunohistochemistry, in situ hybridization, and FACS to analyze the spatial and temporal expression of CD44 and characterize the CD44-positive cells in the mouse cerebellum during development. CD44 expression was observed not only in astrocyte precursor cells but also in neural stem cells and oligodendrocyte precursor cells (OPCs) at early postnatal stages. CD44 expression in OPCs was shut off during oligodendrocyte differentiation. Interestingly, during development, CD44 expression was limited specifically to Bergmann glia and fibrous astrocytes among three types of astrocytes in cerebellum, and expression in astrocytes was shut off during postnatal development. CD44 expression was also detected in developing Purkinje and granule neurons but was limited to granule neurons in the adult cerebellum. Thus, at early developmental stages of the cerebellum, CD44 was widely expressed in several types of precursor cells, and over the course of development, the expression of CD44 became restricted to granule neurons in the adult.
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影响因子：
6.2
作者：
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通讯作者：
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通讯作者：
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