Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
Association between serum haptoglobin and carotid arterial functions: usefulness of a targeted metabolomics approach
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血清触珠蛋白与颈动脉功能之间的关联：靶向代谢组学方法的有用性
DOI：
10.1186/s12933-019-0808-2
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发表时间：
2019-01
影响因子：
9.3
通讯作者：
Jia Weiping
中科院分区：
文献类型：
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作者：
Wang Shiyun;Wang Jie;Zhang Rong;Zhao Aihua;Zheng Xiaojiao;Yan D;an;Jiang Feng;Jia Wei;Hu Cheng;Jia Weiping
背景血清结合珠蛋白（haptoglobin，Hp）与心脑血管疾病密切相关。我们调查了代谢谱与循环Hp和颈动脉功能通过有针对性的代谢组学方法，以提供深入了解潜在的mechanism.MethodsA共240名参与者，包括120例2型糖尿病（T2 DM）和120例非糖尿病（非DM）的主题被招募在这项研究中。使用AbsoluteIDQ™ p180试剂盒（BIOCRATES Life Sciences AG，因斯布鲁克，奥地利）测定血清代谢物的靶向代谢谱。超声测量双侧颈总动脉内膜-中层厚度和外膜间直径。结果2型糖尿病患者和非糖尿病患者血清Hp水平分别为10 3.40（72.46，131.99）mg/dL和10 0.2 0（5 3.99，140.6 6）mg/dL; 2型糖尿病患者和非糖尿病患者血清Hp三分位数中分别有19种和17种代谢产物存在显著性差异（P< 0.05）。其中，磷脂酰胆碱酰基烷基C32：2（PC ae C32：2）是两个人群的共同代谢产物，且与血清Hp分型和血脂性状相关（P< 0.05）。两组人群中天冬氨酸/PC ae C32：2（Asp/PC ae C32：2）和谷氨酸/PC ae C32：2（Glu/PC ae C32：2）的代谢比值与血清Hp、颈动脉功能及其他生化指标显著相关（P<0. 05）。
BackgroundSerum haptoglobin (Hp) has been closely associated with cardio-cerebrovascular diseases. We investigated a metabolic profile associated with circulating Hp and carotid arterial functions via a targeted metabolomics approach to provide insight into potential mechanisms.MethodsA total of 240 participants, including 120 patients with type 2 diabetes mellitus (T2DM) and 120 non-diabetes mellitus (non-DM) subjects were recruited in this study. Targeted metabolic profiles of serum metabolites were determined using an AbsoluteIDQ™ p180 Kit (BIOCRATES Life Sciences AG, Innsbruck, Austria). Ultrasound of the bilateral common carotid artery was used to measure intima-media thickness and inter-adventitial diameter. Serum Hp levels were tested by enzyme-linked immunosorbent assay.ResultsSerum Hp levels in T2DM patients and non-DM subjects were 103.40 (72.46, 131.99) mg/dL and 100.20 (53.99, 140.66) mg/dL, respectively. Significant differences of 19 metabolites and 17 metabolites were found among serum Hp tertiles in T2DM patients and non-DM subjects, respectively (P< 0.05). Of these, phosphatidylcholine acyl-alkyl C32:2 (PC ae C32:2) was the common metabolite observed in two populations, which was associated with the serum Hp groups and lipid traits (P< 0.05). Furthermore, the metabolite ratios of two acidic amino acids, including aspartate to PC ae C32:2 (Asp/PC ae C32:2) and glutamate to PC ae C32:2 (Glu/PC ae C32:2) were correlated with serum Hp, carotid arterial functions and other biochemical index in both populations significantly (P< 0.05).ConclusionsTargeted metabolomics analyses might provide a new insight into the potential mechanisms underlying the association between serum Hp and carotid arterial functions.
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影响因子：
3
作者：
Petersen AK;Krumsiek J;Wägele B;Theis FJ;Wichmann HE;Gieger C;Suhre K
通讯作者：
Suhre K
影响因子：
0.4
作者：
Agnieszka Klupczyńska;Paweł Dereziński;Z. Kokot
通讯作者：
Agnieszka Klupczyńska;Paweł Dereziński;Z. Kokot
影响因子：
5.3
作者：
Melander O;Modrego J;Zamorano-León JJ;Santos-Sancho JM;Lahera V;López-Farré AJ
通讯作者：
López-Farré AJ
影响因子：
9.3
作者：
Katakami N;Mita T;Irie Y;Takahara M;Matsuoka TA;Gosho M;Watada H;Shimomura I;Sitagliptin Preventive study of Intima-media thickness Evaluation (SPIKE) Collaborators
通讯作者：
Sitagliptin Preventive study of Intima-media thickness Evaluation (SPIKE) Collaborators
影响因子：
30.8
作者：
通讯作者：
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