Challenges in the use of sortase and other peptide ligases for site-specific protein modification.
Challenges in the use of sortase and other peptide ligases for site-specific protein modification.
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DOI：
10.1039/d0cs01148g
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发表时间：
2022-05-23
影响因子：
46.2
通讯作者：
中科院分区：
文献类型：
--
作者：
位点特异性蛋白修饰是一种广泛使用的生化工具。然而，与蛋白质修饰技术的发展相关的许多挑战，特别是实现位点特异性，反应效率和多功能性。肽连接酶及其底物的工程已被用于解决这些挑战。本文将重点介绍枯草菌素的分选酶、肽基天冬酰胺连接酶（PALs）和变体；详细介绍了它们的固有特异性如何被用于位点特异性蛋白质修饰。这篇综述将探讨这些酶和底物的工程是如何通过增强催化活性和降低可逆性来提高反应效率的。它还将描述工程肽连接酶如何扩大其底物范围，为扩大生化工具包开辟了新的机会，特别是通过开发使用正交肽连接酶将多个底物位点特异性地偶联到蛋白质上的技术。我们强调化学和生化策略采取优化肽和蛋白质修饰使用肽连接酶。
Site-specific protein modification is a widely-used biochemical tool. However, there are many challenges associated with the development of protein modification techniques, in particular, achieving site-specificity, reaction efficiency and versatility. The engineering of peptide ligases and their substrates has been used to address these challenges. This review will focus on sortase, peptidyl asparaginyl ligases (PALs) and variants of subtilisin; detailing how their inherent specificity has been utilised for site-specific protein modification. The review will explore how the engineering of these enzymes and substrates has led to increased reaction efficiency mainly due to enhanced catalytic activity and reduction of reversibility. It will also describe how engineering peptide ligases to broaden their substrate scope is opening up new opportunities to expand the biochemical toolkit, particularly through the development of techniques to conjugate multiple substrates site-specifically onto a protein using orthogonal peptide ligases. We highlight chemical and biochemical strategies taken to optimise peptide and protein modification using peptide ligases.
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影响因子：
4.7
作者：
Cong, Min;Tavakolpour, Soheil;Berland, Lea;Glockner, Hannah;Andreiuk, Bohdan;Rakhshandehroo, Taha;Uslu, Safak;Mishra, Shruti;Clark, Louise;Rashidian, Mohammad
通讯作者：
Rashidian, Mohammad
影响因子：
2.9
作者：
ABRAHMSEN, L;TOM, J;WELLS, JA
通讯作者：
WELLS, JA
影响因子：
4.8
作者：
Budzik, Jonathan M.;Oh, So-Young;Schneewind, Olaf
通讯作者：
Schneewind, Olaf
影响因子：
--
作者：
Bernath-Levin, Kalia;Nelson, Clark;Mylne, Joshua S.
通讯作者：
Mylne, Joshua S.
影响因子：
3.7
作者：
Beerli RR;Hell T;Merkel AS;Grawunder U
通讯作者：
Grawunder U