Encapsulation of hydrophobic components in dendrimersomes and decoration of their surface with proteins and nucleic acids
Encapsulation of hydrophobic components in dendrimersomes and decoration of their surface with proteins and nucleic acids
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将疏水性成分封装在树枝状聚合物中并用蛋白质和核酸修饰其表面
DOI：
10.1073/pnas.1904868116
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Klein, Michael L.
中科院分区：
文献类型：
--
作者：
Torre, Paola;Xiao, Qi;Buzzacchera, Irene;Sherman, Samuel E.;Rahimi, Khosrow;Kostina, Nina Yu.;Rodriguez-Emmenegger, Cesar;Möller, Martin;Wilson, Christopher J.;Klein, Michael L.
Reconstructing the functions of living cells using nonnatural components is one of the great challenges of natural sciences. Compartmentalization, encapsulation, and surface decoration of globular assemblies, known as vesicles, represent key early steps in the reconstitution of synthetic cells. Here we report that vesicles self-assembled from amphiphilic Janus dendrimers, called dendrimersomes, encapsulate high concentrations of hydrophobic components and do so more efficiently than commercially available stealth liposomes assembled from phospholipid components. Multilayer onion-like dendrimersomes demonstrate a particularly high capacity for loading low-molecular weight compounds and even folded proteins. Coassembly of amphiphilic Janus dendrimers with metal-chelating ligands conjugated to amphiphilic Janus dendrimers generates dendrimersomes that selectively display folded proteins on their periphery in an oriented manner. A modular strategy for tethering nucleic acids to the surface of dendrimersomes is also demonstrated. These findings augment the functional capabilities of dendrimersomes to serve as versatile biological membrane mimics.
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DOI：
10.1073/pnas.1821924116
发表时间：
2019-02
影响因子：
11.1
作者：
César Rodriguez-Emmenegger;Qi Xiao;N. Kostina;Samuel E Sherman;K. Rahimi;Benjamin E. Partridge;Shangda Li;Dipankar Sahoo;Aracelee M. Reverón Pérez;Irene Buzzacchera;Hong Han;Meir Kerzner;I. Malhotra;M. Möller;Christopher J. Wilson;Matthew C. Good;M. Goulian;T. Baumgart;M. Klein;V. Percec
通讯作者：
César Rodriguez-Emmenegger;Qi Xiao;N. Kostina;Samuel E Sherman;K. Rahimi;Benjamin E. Partridge;Shangda Li;Dipankar Sahoo;Aracelee M. Reverón Pérez;Irene Buzzacchera;Hong Han;Meir Kerzner;I. Malhotra;M. Möller;Christopher J. Wilson;Matthew C. Good;M. Goulian;T. Baumgart;M. Klein;V. Percec
影响因子：
2.9
作者：
Paborsky, LR;Dunn, KE;Dougherty, JP
通讯作者：
Dougherty, JP
影响因子：
15
作者：
Xiao Q;Rubien JD;Wang Z;Reed EH;Hammer DA;Sahoo D;Heiney PA;Yadavalli SS;Goulian M;Wilner SE;Baumgart T;Vinogradov SA;Klein ML;Percec V
通讯作者：
Percec V
影响因子：
56.9
作者：
Discher, BM;Won, YY;Hammer, DA
通讯作者：
Hammer, DA
新型可聚合金属螯合脂质的合成。
影响因子：
3.6
作者：
B. Roy;S. Mallik
通讯作者：
S. Mallik