Direct evidence for heme-assisted solid-state electronic conduction in multi-heme c-type cytochromes.
Direct evidence for heme-assisted solid-state electronic conduction in multi-heme c-type cytochromes.
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多血红素 C 型细胞色素中血红素辅助固态电子传导的直接证据
DOI：
10.1039/c8sc01716f
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发表时间：
2018-10-07
期刊：
影响因子：
8.4
通讯作者：
Cahen D
中科院分区：
文献类型：
--
作者：
Garg K;Ghosh M;Eliash T;van Wonderen JH;Butt JN;Shi L;Jiang X;Zdenek F;Blumberger J;Pecht I;Sheves M;Cahen D
我们研究了两个多血红素细胞色素c型蛋白质，MtrF和STC的无溶剂电子传输，并发现它们比非或单血红素蛋白质更好地进行。多血红素细胞色素c（Cytc）蛋白是将电子转移出细胞的关键，使细胞内氧化能够在没有O2的情况下进行。在这些蛋白质中，大多数血红素以线性阵列排列，表明电子传导的简易路径。为了测试这一点，我们研究了两种多血红素Cytc型蛋白质的无溶剂电子传递：MtrF（十血红素Cytc）和STC（四血红素Cytc）。运输测量这些蛋白质在Au电极之间的固态配置的单层。这两种蛋白质的电导率比单一血红素或无血红素蛋白质高1000倍，但与共轭有机物单层的电导率相似。发现电导与温度无关（320-80 K），表明隧穿是传输机制。该机制与I-V曲线建模一致，其结果可以通过将蛋白质-电极耦合作为速率限制而不是蛋白质内的运输来解释。
We study solvent-free electron transport across two multi-heme cytochrome c-type proteins, MtrF and STC, and find that they are better at conducting than non- or mono heme proteins. Multi-heme cytochrome c (Cytc) proteins are key for transferring electrons out of cells, to enable intracellular oxidation to proceed in the absence of O2. In these proteins most of the hemes are arranged in a linear array suggesting a facile path for electronic conduction. To test this, we studied solvent-free electron transport across two multi-heme Cytc-type proteins: MtrF (deca-heme Cytc) and STC (tetra-heme Cytc). Transport is measured across monolayers of these proteins in a solid state configuration between Au electrodes. Both proteins showed 1000× higher conductance than single heme, or heme-free proteins, but similar conductance to monolayers of conjugated organics. Conductance is found to be temperature-independent (320–80 K), suggesting tunneling as the transport mechanism. This mechanism is consistent with I–V curves modelling, results of which could be interpreted by having protein-electrode coupling as rate limiting, rather than transport within the proteins.
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影响因子：
4.4
作者：
LOVLEY, DR;PHILLIPS, EJP
通讯作者：
PHILLIPS, EJP
影响因子：
15
作者：
Sepunaru, Lior;Pecht, Israel;Cahen, David
通讯作者：
Cahen, David
DOI：
10.1073/pnas.1316156111
发表时间：
2014-01-14
影响因子：
11.1
作者：
Breuer, Marian;Rosso, Kevin M.;Blumberger, Jochen
通讯作者：
Blumberger, Jochen
影响因子：
4.6
作者：
Edwards MJ;White GF;Norman M;Tome-Fernandez A;Ainsworth E;Shi L;Fredrickson JK;Zachara JM;Butt JN;Richardson DJ;Clarke TA
通讯作者：
Clarke TA
影响因子：
3.4
作者：
Polizzi NF;Skourtis SS;Beratan DN
通讯作者：
Beratan DN