The Saccharomyces cerevisiae small GTPase, Gsp1p/Ran, is involved in 3' processing of 7S-to-5.8S rRNA and in degradation of the excised 5'-A0 fragment of 35S pre-rRNA, both of which are carried out by the exosome.
The Saccharomyces cerevisiae small GTPase, Gsp1p/Ran, is involved in 3' processing of 7S-to-5.8S rRNA and in degradation of the excised 5'-A0 fragment of 35S pre-rRNA, both of which are carried out by the exosome.
复制标题
酿酒酵母小 GTP 酶 Gsp1p/Ran 参与 7S 至 5.8S rRNA 的 3 加工以及 35S pre-rRNA 切除的 5-A0 片段的降解，这两者均由外泌体进行
作者：
N. Suzuki;E. Noguchi;N. Nakashima;M. Oki;T. Ohba;A. Tartakoff;M. Ohishi;T. Nishimoto
Dis3p, a subunit of the exosome, interacts directly with Ran. To clarify the relationship between the exosome and the RanGTPase cycle, a series of temperature-sensitive Saccharomyces cerevisiae dis3 mutants were isolated and their 5.8S rRNA processing was compared with processing in strains with mutations in a S. cerevisiae Ran homologue, Gsp1p. In both dis3 and gsp1 mutants, 3' processing of 7S-to-5.8S rRNA was blocked at three identical sites in an allele-specific manner. In contrast, the 5' end of 5.8S rRNA was terminated normally in gsp1 and in dis3. Inhibition of 5.8S rRNA maturation in gsp1 was rescued by overexpression of nuclear exosome components Dis3p, Rrp4p, and Mtr4p, but not by a cytoplasmic exosome component, Ski2p. Furthermore, gsp1 and dis3 accumulated the 5'-A0 fragment of 35S pre-rRNA, which is also degraded by the exosome, and the level of 27S rRNA was reduced. Neither 5.8S rRNA intermediates nor 5'-A0 fragments were observed in mutants defective in the nucleocytoplasmic transport, indicating that Gsp1p regulates rRNA processing through Dis3p, independent of nucleocytoplasmic transport.
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影响因子：
2.9
作者：
BECKMAN, RA;MILDVAN, AS;LOEB, LA
通讯作者：
LOEB, LA
影响因子：
56.9
作者：
SACHS, AB;DAVIS, RW
通讯作者：
DAVIS, RW
小核 RNA 前体的核质运输和加工。
影响因子：
5.3
作者：
NeumandeVegvar,HE;Dahlberg,JE
通讯作者：
Dahlberg,JE
酵母 Srp1p 与犰狳/斑球蛋白/β-连环蛋白具有同源性，并参与明显的多种核功能，包括维持核仁结构。
DOI：
10.1073/pnas.91.15.6880
发表时间：
1994
影响因子：
11.1
作者：
Yano,R;Oakes,ML;Tabb,MM;Nomura,M
通讯作者：
Nomura,M