Gracilimonas mengyeensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-western China.
Gracilimonas mengyeensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-western China.
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孟江纤细单胞菌 sp.
DOI：
10.1099/ijs.0.052043-0
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发表时间：
2013-11
影响因子：
2.8
通讯作者：
Cui Xxiao-Long
中科院分区：
文献类型：
--
作者：
Li Zhi-Ying;Ding Zhang-Gui;Wen Meng-Lang;Cui Xxiao-Long
从云南某盐矿沉积物中分离到一株兼性厌氧、革兰氏染色阴性、淡红色、不活动、棒状、中度嗜盐细菌，命名为YIM J14(T)。当NaCl浓度为2% ~ 15% （w/v）时，生长最快，当NaCl浓度为5 ~ 9%时生长最快。菌株生长的最佳温度为28℃，pH为7.5。主要细胞脂肪酸为iso-C15: 0、前iso-C15: 0和iso-C17: 1ω9c/10-甲基- c16: 0。极性脂质谱主要由二磷脂酰甘油、磷脂酰胆碱和一种未知的磷脂组成。少量的其他脂质也被检测到。基因组DNA G+C含量为47.2% mol%。基于16S rRNA基因序列比较的系统发育分析表明，菌株YIM J14(T)与拟杆菌门的Gracilimonas tropica有亲缘关系。菌株YIM J14(T)与热带Gracilimonas tropical CL-CB462(T)的16S rRNA基因序列相似性水平为96.9%。菌株YIM J14(T)与热带Gracilimonas tropica的DNA-DNA杂交实验表明亲缘度为28%。化学分类数据支持菌株YIM J14(T)在Gracilimonas属中的定位。通过DNA-DNA杂交和生化生理鉴定，将YIM J14(T)从Gracilimonas tropica中分离出来。因此，它被认为是Gracilimonas属的一个新种，因此建议将其命名为Gracilimonas mengyeensis sp. nov。型应变YIM J14(T) (= ACCC 10717（T) = DSM 21985(T)）。
A facultatively anaerobic, Gram-staining-negative, pale red-pigmented, non-motile, rod-shaped, moderately halophilic bacterium, designated strain YIM J14(T), was isolated from a sediment sample from a salt mine in Yunnan, south-western China. Growth occurred at NaCl concentrations of between 2 % and 15 % (w/v) and optimally with 5-9 % NaCl. The optimum temperature and pH for growth of the strain were 28 °C and pH 7.5. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 1ω9c/10-methyl-C16 : 0. The polar lipid profile was composed predominantly of diphosphatidylglycerol, phosphatidylcholine and one unknown phospholipid. Minor amounts of other lipids were also detectable. The genomic DNA G+C content was 47.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain YIM J14(T) was related to Gracilimonas tropica in the phylum Bacteroidetes. The level of 16S rRNA gene sequence similarity between strain YIM J14(T) and Gracilimonas tropica CL-CB462(T) was 96.9 %. A DNA-DNA hybridization experiment between strain YIM J14(T) and Gracilimonas tropica indicated levels of relatedness of 28 %. Chemotaxonomic data supported the placement of strain YIM J14(T) in the genus Gracilimonas. DNA-DNA hybridization and biochemical and physiological characterization allowed strain YIM J14(T) to be differentiated from Gracilimonas tropica. It is therefore considered to represent a novel species of the genus Gracilimonas, for which the name Gracilimonas mengyeensis sp. nov. is proposed. The type strain YIM J14(T) ( = ACCC 10717(T) = DSM 21985(T)).
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影响因子：
64.8
作者：
KOVACS, N
通讯作者：
KOVACS, N
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
影响因子：
4.4
作者：
Allgaier, M;Uphoff, H;Wagner-Döbler, I
通讯作者：
Wagner-Döbler, I
DOI：
10.1016/s0176-6724(88)80120-2
发表时间：
1988-03
期刊：
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology
影响因子：
--
作者：
Lawrence G. Wayne
通讯作者：
Lawrence G. Wayne
影响因子：
6.5
作者：
W. Fitch
通讯作者：
W. Fitch