Protocadherin-19 and N-cadherin interact to control cell movements during anterior neurulation.
Protocadherin-19 and N-cadherin interact to control cell movements during anterior neurulation.
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DOI：
10.1083/jcb.201007008
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发表时间：
2010-11-29
期刊：
影响因子：
--
通讯作者：
Jontes JD
中科院分区：
文献类型：
--
作者：
Biswas S;Emond MR;Jontes JD
原钙粘蛋白19和N-钙粘蛋白在斑马鱼脑形态发生中协同作用。原钙粘蛋白是钙粘蛋白超家族中最大的一个亚类，但其细胞和发育功能尚不清楚。在这项研究中，我们证明了pcdh 19（原钙粘蛋白19）与n-cadherin（ncad）在斑马鱼前神经胚形成过程中的协同作用。此外，Pcdh 19和Ncad直接相互作用，在体外和体内形成蛋白质-蛋白质复合物。虽然这两种分子都需要在斑马鱼细胞系中的钙依赖性粘附，Pcdh 19的胞外结构域不表现出粘附活性，这表明Pcdh 19参与细胞粘附是间接的。对体内双光子延时成像序列的定量分析表明，pcdh 19或ncad的缺失会损害神经形成期间的细胞运动，破坏细胞运动的方向性和相邻细胞之间运动的一致性。我们的研究结果表明，Pcdh 19和Ncad功能一起调节细胞粘附，并介导在大脑发育过程中的形态发生运动。
Protocadherin-19 and N-cadherin act synergistically during brain morphogenesis in zebrafish. The protocadherins comprise the largest subgroup within the cadherin superfamily, yet their cellular and developmental functions are not well understood. In this study, we demonstrate that pcdh19 (protocadherin 19) acts synergistically with n-cadherin (ncad) during anterior neurulation in zebrafish. In addition, Pcdh19 and Ncad interact directly, forming a protein–protein complex both in vitro and in vivo. Although both molecules are required for calcium-dependent adhesion in a zebrafish cell line, the extracellular domain of Pcdh19 does not exhibit adhesive activity, suggesting that the involvement of Pcdh19 in cell adhesion is indirect. Quantitative analysis of in vivo two-photon time-lapse image sequences reveals that loss of either pcdh19 or ncad impairs cell movements during neurulation, disrupting both the directedness of cell movements and the coherence of movements among neighboring cells. Our results suggest that Pcdh19 and Ncad function together to regulate cell adhesion and to mediate morphogenetic movements during brain development.
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DOI：
10.1126/science.1167094
发表时间：
2008-12-05
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
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通讯作者：
Stathopoulos A
影响因子：
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作者：
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通讯作者：
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影响因子：
9.9
作者：
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通讯作者：
Guerrini, R.
影响因子：
2.7
作者：
Emond, Michelle R.;Jontes, James D.
通讯作者：
Jontes, James D.
影响因子：
4.6
作者：
Geldmacher-Voss, B;Reugels, AM;Campos-Ortega, JA
通讯作者：
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