Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy.
Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy.
复制标题
通过冷冻扫描电子显微镜可视化木质组织中水分分布的冷冻平面技术。
DOI：
10.1007/978-1-59745-294-6_24
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发表时间：
2007
影响因子：
--
通讯作者：
Y. Sano
中科院分区：
文献类型：
--
作者：
Yasuhiro Utsumi;Y. Sano
本文介绍了利用低温扫描电镜技术研究活体树干水分分布的低温刨平技术。简单地说，程序如下：首先，一部分蒸腾茎在静置状态下用液氮冷冻，以稳定传导组织中存在的水。用液氮填充后，然后从茎的冷冻部分收集圆盘并储存在液氮中。在-20 °C的低温室中使用滑动切片机干净地切割椎间盘表面。最后，冷冻样品在冷冻蚀刻和金属涂层后在冷冻扫描电子显微镜中进行检查。
The protocol of cryoplaning techniques that to examine the distribution of water in living tree stems by cryoscanning electron microscopy have been developed and described. In brief, the procedures are as follows: First, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. The surface of disc is cleanly cut using a sliding microtome in a low temperature room at −20°C. Finally, the frozen sample is examined in a cryoscanning electron microscope after freeze-etching and metal coating.
影响因子：
4
作者：
Sano, Y;Okamura, Y;Utsumi, Y
通讯作者：
Utsumi, Y
影响因子：
7.4
作者：
Kuroda, K;Kasuga, J;Fujikawa, S
通讯作者：
Fujikawa, S