Functional genomics reveals increases in cholesterol biosynthetic genes and highly unsaturated fatty acid biosynthesis after dietary substitution of fish oil with vegetable oils in Atlantic salmon (Salmo salar).
Functional genomics reveals increases in cholesterol biosynthetic genes and highly unsaturated fatty acid biosynthesis after dietary substitution of fish oil with vegetable oils in Atlantic salmon (Salmo salar).
复制标题
DOI：
10.1186/1471-2164-9-299
复制
发表时间：
2008-06-24
期刊：
影响因子：
4.4
通讯作者：
Taggart JB
中科院分区：
文献类型：
--
作者：
Leaver MJ;Villeneuve LA;Obach A;Jensen L;Bron JE;Tocher DR;Taggart JB
由于来自野生鱼类资源的鱼油供应短缺，越来越多的人希望用植物油（VO）取代鱼类养殖饲料中的鱼油（FO）。然而，很少有人知道这种替代后对鱼类健康的影响。饮食VO对肝脏基因表达，脂质组成和生长的影响，确定在大西洋鲑鱼（萨尔莫salar），使用基因芯片，脂质和生化分析相结合。试验用VO代替FO，以菜籽油、豆油和亚麻籽油添加到日粮中。饲料中添加VO对鱼的生长没有显著影响，但能提高全鱼蛋白质含量，降低全鱼脂肪含量，从而提高蛋白质与脂肪的比值。在所有植物油饮食中，高度不饱和脂肪酸（HUFA）和胆固醇生物合成途径的基因表达水平增加，SREBP 2也是这些途径的主要转录调节因子。其他基因的表达增加喂养VO包括NADPH生成，脂质转运，过氧化物酶体脂肪酸氧化，细胞内脂质积累的标志物，蛋白质和RNA加工。与这些结果一致，VO改变了HUFA的生物合成、肝脏β-氧化活性和酶促NADPH的产生，LO和SO日粮中有增加肝脏脂质的趋势。组织胆固醇水平在VO喂养的鱼是相同的动物喂养的FO，而脂肪酸组成的组织在很大程度上反映了那些的饮食和标记的富集18碳脂肪酸和减少20和22碳HUFA。这种组合的基因表达，组成和代谢研究表明，主要的脂质代谢的影响发生后，取代FO与VO鲑鱼饮食。这些作用最有可能是由SREBP 2介导的，SREBP 2对膳食胆固醇的降低有反应。这些变化足以维持全身胆固醇水平，但不能维持HUFA水平。
There is an increasing drive to replace fish oil (FO) in finfish aquaculture diets with vegetable oils (VO), driven by the short supply of FO derived from wild fish stocks. However, little is known of the consequences for fish health after such substitution. The effect of dietary VO on hepatic gene expression, lipid composition and growth was determined in Atlantic salmon (Salmo salar), using a combination of cDNA microarray, lipid, and biochemical analysis. FO was replaced with VO, added to diets as rapeseed (RO), soybean (SO) or linseed (LO) oils. Dietary VO had no major effect on growth of the fish, but increased the whole fish protein contents and tended to decrease whole fish lipid content, thus increasing the protein:lipid ratio. Expression levels of genes of the highly unsaturated fatty acid (HUFA) and cholesterol biosynthetic pathways were increased in all vegetable oil diets as was SREBP2, a master transcriptional regulator of these pathways. Other genes whose expression was increased by feeding VO included those of NADPH generation, lipid transport, peroxisomal fatty acid oxidation, a marker of intracellular lipid accumulation, and protein and RNA processing. Consistent with these results, HUFA biosynthesis, hepatic β-oxidation activity and enzymic NADPH production were changed by VO, and there was a trend for increased hepatic lipid in LO and SO diets. Tissue cholesterol levels in VO fed fish were the same as animals fed FO, whereas fatty acid composition of the tissues largely reflected those of the diets and was marked by enrichment of 18 carbon fatty acids and reductions in 20 and 22 carbon HUFA. This combined gene expression, compositional and metabolic study demonstrates that major lipid metabolic effects occur after replacing FO with VO in salmon diets. These effects are most likely mediated by SREBP2, which responds to reductions in dietary cholesterol. These changes are sufficient to maintain whole body cholesterol levels but not HUFA levels.
登录
查看更多内容
影响因子：
2.9
作者：
Bell, JG;Tocher, DR;Sargent, JR
通讯作者：
Sargent, JR
影响因子：
4.2
作者：
Diez, Amalia;Menoyo, David;Bautista, Jose M.
通讯作者：
Bautista, Jose M.
影响因子：
3.5
作者：
Mourente, G;Good, JE;Bell, JG
通讯作者：
Bell, JG
影响因子：
4.6
作者：
Morais, Sofia;Knoll-Gellida, Anja;Babin, Patrick J.
通讯作者：
Babin, Patrick J.
DOI：
10.1073/pnas.1534923100
发表时间：
2003-10-14
影响因子：
11.1
作者：
Horton, JD;Shah, NA;Goldstein, JL
通讯作者：
Goldstein, JL