Role of the MAPK/cJun NH(2)-terminal kinase signaling pathway in starvation-induced autophagy.
Role of the MAPK/cJun NH(2)-terminal kinase signaling pathway in starvation-induced autophagy.
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DOI：
10.1080/15548627.2018.1466013
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发表时间：
2018
期刊：
影响因子：
13.3
通讯作者：
Davis RJ
中科院分区：
文献类型：
--
作者：
Barutcu SA;Girnius N;Vernia S;Davis RJ
自噬是细胞稳态所必需的，并且可以决定细胞响应于应激的活力。已经确定MTOR是饥饿诱导的巨自噬/自噬的主要调节剂，但最近的研究还暗示了MAPK 8/cJun NH 2-末端激酶1信号转导途径的重要作用。我们发现MAPK 8/JNK 1和MAPK 9/JNK 2在小鼠成纤维细胞和上皮细胞中不需要饥饿或MTOR抑制引起的自噬。这些数据表明MAPK 8/9在饥饿诱导的自噬中没有必要的作用。我们的结论是MAPK 8/9在自噬中的作用可能是上下文依赖的，比以前认为的更复杂。缩略语：AKT：胸腺瘤病毒原癌基因; ALB：白蛋白; ATG4：自噬相关4例; BCL 2：B细胞白血病/淋巴瘤2; BECN 1：beclin 1，自噬相关; BNIP 3：BCL 2/腺病毒E1 B相互作用蛋白3; CQ：二磷酸氯喹; DMEM：Dulbecco改良Eagle培养基; EDTA：乙二胺四乙酸; EBSS：Earle平衡盐溶液; FBS：胎牛血清; GAPDH：甘油醛-3-磷酸脱氢酶; GFP：绿色荧光蛋白; HRAS：Harvey大鼠肉瘤病毒癌基因; IgG：免疫球蛋白G; MAPK 3/ERK 1：促分裂原活化蛋白激酶3; MAPK 8/JNK 1：促分裂原活化蛋白激酶8; MAPK 9/JNK 2：促分裂原活化蛋白激酶9; MAPK 10/JNK 3：促分裂原活化蛋白激酶10; MAP 1 LC 3B/LC 3B：微管相关蛋白1轻链3 β; MEF：小鼠胚胎成纤维细胞; MTOR：雷帕霉素激酶的机制靶点; RPS 6 KB 1/p70：核糖体蛋白S6激酶，多肽1; PPARA：过氧化物酶体增殖物激活受体α; SEM：平均值的标准误差; SQSTM 1/p62：多价螯合体1; TORC 1：雷帕霉素复合物1的靶标; TORC 2：雷帕霉素复合物2的靶标; TRP 53：转化相关蛋白53; TUBA：微管蛋白α; UV：紫外线; WT：野生型
Autophagy is required for cellular homeostasis and can determine cell viability in response to stress. It is established that MTOR is a master regulator of starvation-induced macroautophagy/autophagy, but recent studies have also implicated an essential role for the MAPK8/cJun NH2-terminal kinase 1 signal transduction pathway. We found that MAPK8/JNK1 and MAPK9/JNK2 were not required for autophagy caused by starvation or MTOR inhibition in murine fibroblasts and epithelial cells. These data demonstrate that MAPK8/9 has no required role in starvation-induced autophagy. We conclude that the role of MAPK8/9 in autophagy may be context-dependent and more complex than previously considered. Abbreviations: AKT: thymoma viral proto-oncogene;ALB: albumin; ATG4: autophagy related 4; BCL2: B cell leukemia/lymphoma 2; BECN1: beclin 1, autophagy related; BNIP3: BCL2/adenovirus E1B interacting protein 3; CQ: chloroquine diphosphate; DMEM: Dulbecco’s modified Eagle’s medium; EDTA: ethylenediaminetetraacetic acid; EBSS: Earle’s balanced salt solution; FBS: fetal bovine serum; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFP: green fluorescent protein; HRAS: Harvey rat sarcoma virus oncogene; IgG: Immunoglobulin G; MAPK3/ERK1: mitogen-activated protein kinase 3; MAPK8/JNK1: mitogen-activated protein kinase 8; MAPK9/JNK2: mitogen-activated protein kinase 9; MAPK10/JNK3: mitogen-activated protein kinase 10; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MEFs: mouse embryonic fibroblasts; MTOR: mechanistic target of rapamycin kinase; RPS6KB1/p70: ribosomal protein S6 kinase, polypeptide 1; PPARA: peroxisome proliferator activated receptor alpha; SEM: standard error of the mean; SQSTM1/p62: sequestosome 1; TORC1: target of rapamycin complex 1; TORC2: target of rapamycin complex 2; TRP53: transforming related protein 53; TUBA: tubulin alpha; UV: ultraviolet; WT: wild-type
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影响因子：
64.8
作者：
Lee, Jae Man;Wagner, Martin;Xiao, Rui;Kim, Kang Ho;Feng, Dan;Lazar, Mitchell A.;Moore, David D.
通讯作者：
Moore, David D.
影响因子：
4.8
作者：
Adnan, Mohammad;Malathi, Siddiqui Krishnamurthy
通讯作者：
Malathi, Siddiqui Krishnamurthy
影响因子：
4.7
作者：
Byun, Joo-Yun;Yoon, Chang-Hwan;Lee, Su-Jae
通讯作者：
Lee, Su-Jae
影响因子：
7.3
作者：
Guertin, David A.;Sabatini, David M.
通讯作者：
Sabatini, David M.
影响因子：
4.8
作者：
Boeck, Barbara C.;Tagscherer, Katrin E.;Roth, Wilfried
通讯作者：
Roth, Wilfried