Minimally invasive, longitudinal monitoring of biomaterial-associated inflammation by fluorescence imaging.
Minimally invasive, longitudinal monitoring of biomaterial-associated inflammation by fluorescence imaging.
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DOI：
10.1016/j.biomaterials.2011.07.020
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发表时间：
2011-11
期刊：
影响因子：
14
通讯作者：
Garcia, Andres J.
中科院分区：
文献类型：
--
作者：
Selvam, Shivaram;Kundu, Kousik;Templeman, Kellie L.;Murthy, Niren;Garcia, Andres J.
植入物相关炎症是导致许多医疗器械性能/寿命降低和失效的主要原因。因此，非侵入性和定量监测植入物相关炎症的能力至关重要。在这里，我们表明，植入物相关的炎症可以通过荧光成像使用近红外氢菁染料局部或静脉注射在活小鼠。这种成像策略允许通过检测炎症细胞响应于植入的聚对苯二甲酸乙二醇酯（PET）盘或注射的聚（乳酸-共-乙醇酸）（PLGA）微粒而释放的活性氧（ROS）来定量纵向监测炎症，并表现出与常规炎症分析的强相关性。此外，使用这种灵敏的成像方法检测通过抗炎剂地塞米松的受控释放的炎症反应的调节。因此，ROS的基于氢菁的荧光成像可以作为监测植入物相关炎症以及评估调节宿主对植入医疗器械反应的治疗方法的功效的替代措施。
Implant-associated inflammation is a major cause for the reduced performance/lifetime and failure of numerous medical devices. Therefore, the ability to non-invasively and quantitatively monitor implant-associated inflammation is critically important. Here we show that implant-associated inflammation can be imaged via fluorescence imaging using near-infrared hydrocyanine dyes delivered either locally or intravenously in living mice. This imaging strategy allowed quantitative longitudinal monitoring of inflammation by detecting reactive oxygen species (ROS) released by inflammatory cells in response to implanted poly(ethylene terephthalate) (PET) disks or injected poly (lactic-co-glycolic acid) (PLGA) microparticles, and exhibited a strong correlation to conventional analysis of inflammation. Furthermore, modulation of inflammatory responses via controlled release of the anti-inflammatory agent dexamethasone was detected using this sensitive imaging approach. Thus, hydrocyanine-based fluorescence imaging of ROS could serve as a surrogate measure for monitoring implant-associated inflammation as well as evaluating the efficacy of therapeutic approaches to modulate host responses to implanted medical devices.
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影响因子：
7.4
作者：
Lin, Patricia W.;Myers, Loren E. S.;Ray, Laurie;Song, Shuh-Chyung;Nasr, Tala R.;Berardinelli, Andrew J.;Kundu, Kousik;Murthy, Niren;Hansen, Jason M.;Neish, Andrew S.
通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.0803945105
发表时间：
2008-11-25
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Coimbra, Raul