Differential effect of grape seed extract against human non-small-cell lung cancer cells: the role of reactive oxygen species and apoptosis induction.
Differential effect of grape seed extract against human non-small-cell lung cancer cells: the role of reactive oxygen species and apoptosis induction.
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DOI：
10.1080/01635581.2013.785003
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Agarwal C
中科院分区：
文献类型：
--
作者：
Tyagi A;Raina K;Gangar S;Kaur M;Agarwal R;Agarwal C
Present study examines grape seed extract (GSE) efficacy against a series of Non-small-cell lung cancer (NSCLC) cell lines which differ in their Kras and p53 status to establish GSE potential as a cytotoxic agent against a wide-range of lung cancer cells. GSE suppressed growth and induced apoptotic death in NSCLC cells irrespective of their k-Ras status, with more sensitivity towards H460 and H322 (wt k-Ras) than A549 and H1299 cells (mutated k-Ras). Mechanistic studies in A549 and H460 cells, selected, based on comparative efficacy of GSE at higher and lower doses, respectively, showed that apoptotic death involves cytochrome c release associated caspases 9 and 3 activation, and PARP cleavage, strong phosphorylation of ERK1/2 and JNK1/2, down regulation of cell survival proteins, and up regulated pro-apoptotic Bak expression. Importantly, GSE treatment caused a strong superoxide radical-associated oxidative stress, significantly decreased intracellular reduced glutathione levels, suggesting, for the first-time, the involvement of GSE-caused oxidative stress in its apoptotic inducing activity in these cells. Since GSE is a widely-consumed dietary agent with no known untoward effects, our results support future studies to establish GSE efficacy and usefulness against NSCLC control.
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DOI：
10.1055/s-0030-1255636
发表时间：
2010-09-01
期刊：
Pneumologie (Stuttgart, Germany)
影响因子：
--
作者：
Goeckenjan, G
通讯作者：
Goeckenjan, G
影响因子：
4.2
作者：
Kaur, Manjinder;Agarwal, Chapla;Agarwal, Rajesh
通讯作者：
Agarwal, Rajesh
影响因子：
8
作者：
Kralova, J.;Sheely, J. I.;Bose, H. R., Jr.
通讯作者：
Bose, H. R., Jr.
影响因子：
11.2
作者：
Kumar, Pawan;Coltas, Ila K.;Polverini, Peter J.
通讯作者：
Polverini, Peter J.
影响因子：
--
作者：
Essick EE;Sam F
通讯作者：
Sam F