Detection of serum IgG4 levels in patients with IgG4-related disease and other disorders.
Detection of serum IgG4 levels in patients with IgG4-related disease and other disorders.
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IgG4 相关疾病和其他疾病患者血清 IgG4 水平的检测
DOI：
10.1371/journal.pone.0124233
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Dong L
中科院分区：
文献类型：
--
作者：
Su Y;Sun W;Wang C;Wu X;Miao Y;Xiong H;Bai L;Dong L
目的血清IgG 4水平升高是诊断IgG 4相关疾病（IgG 4-RD）的重要标志，但也可在其他疾病中观察到。本研究旨在比较两种不同的IgG 4检测方法：ELISA法和散射比浊法。两种测定法均用于测量血清IgG 4浓度，并评估IgG 4-RD和非IgG 4-RD疾病中高血清IgG 4水平的患病率。方法采用自行建立的散射比浊法和ELISA法对80份血清标本进行检测。用ELISA法测定957例不同疾病患者血清IgG_4浓度，其中IgG_4-RD患者12例，非IgG_4-RD患者945例。结果80份血清标本的IgG 4水平ELISA检测结果与散射比浊法检测结果一致。同时，ELISA法测定的血清IgG 4浓度也与病程中IgG 4-RD患者的临床诊断相一致。12例IgG 4-RD患者血清IgG 4水平均升高（> 1.35g/L），非IgG 4-RD患者中IgG 4水平升高的发生率为3.39%。其中，恶性肿瘤患者血清IgG 4阳性率为2.06%，其他非IgG 4自身免疫性疾病患者为6.3%。结论建立的ELISA方法简便、可靠，可广泛应用于临床。在IgG 4-RD中观察到高水平的IgG 4。然而，这种现象也可以在其他疾病中观察到，例如癌症和其他自身免疫性疾病。因此，IgG 4疾病的诊断不能仅依赖于血清IgG 4水平升高的检测。
Objective Elevated serum IgG4 levels are an important hallmark for diagnosing IgG4-related disease (IgG4-RD), but can also be observed in other diseases. This study aimed to compare two different testing methods for IgG4: ELISA and nephelometric assay. Both assays were used to measure serum IgG4 concentrations, and to assess the prevalence of high serum IgG4 levels in both IgG4-RD and non-IgG4-RD diseases. Methods A total of 80 serum samples were tested using the nephelometric assay and ELISA method that we established. Serum IgG4 concentrations were determined by ELISA for 957 patients with distinct diseases, including 12 cases of IgG4-RD and 945 cases of non-IgG4-RD. Results IgG4 levels from 80 selected serum samples examined by ELISA were in agreement with those detected using the nephelometry assay. Meanwhile, the serum IgG4 concentrations measured by ELISA were also consistent with the clinical diagnoses of patients with IgG4-RD during the course of disease. The Elevated levels of serum IgG4 (>1.35 g/L) were detected in all IgG4-RD (12/12) patients, and the prevalence of high IgG4 serum levels was 3.39% in non-IgG4-RD cases. Among them, the positive rates of serum IgG4 were 2.06% in patients with carcinoma and 6.3% in patients with other non-IgG4 autoimmune diseases. Conclusion Our established ELISA method is a reliable and convenient technique, which could be extensively used in the clinic to measure serum IgG4 levels. High levels of IgG4 were observed in IgG4-RD. However, this phenomenon could also be observed in other diseases, such as carcinomas and other autoimmune diseases. Thus, a diagnosis of IgG4 disease cannot only be dependent on the detection of elevated serum IgG4 levels.
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影响因子：
2.3
作者：
Okazaki K;Umehara H
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