Cable Properties of Dendrites in Hippocampal Neurons of the Rat Mapped by a Voltage‐sensitive Dye
Cable Properties of Dendrites in Hippocampal Neurons of the Rat Mapped by a Voltage‐sensitive Dye
复制标题
电压敏感染料绘制的大鼠海马神经元树突的电缆特性
DOI：
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发表时间：
1997
影响因子：
3.4
通讯作者：
P. Fromherz
中科院分区：
文献类型：
--
作者：
E. Meyer;C. O. Müller;P. Fromherz
使用电压敏感性荧光染料在培养物中研究来自胚胎大鼠海马的锥体神经元的树突。对躯体刺激的电反应被观察为时间分辨图，分辨率为0.9 μ M，时间常数为0.4 ms，无信号平均。这些数据是用Hodgkin-Huxley参数化的锥形电缆来解释的。短超极化瞬变的传播被电容分流阻尼。动作电位的侵入由低密度的电压门控电导增强。在分叉处未观察到不规则性。在静息电压下，无源电缆的内阻和膜电阻参数分别为Ri = 300 ω cm和Rm = 40 k ω cm 2，最大钠电导为104.4 mS/cm 2。在1kHz处的电张长度常数和动态长度常数分别为580和90 μ m。这些结果与成年海马切片中树突的电生理数据和培养中水蛭神经元窄突起的光学数据是一致的。考虑了通过低密度的电压门控通道提高动作电位的功能意义。
Dendrites of pyramidal neurons from embryonic rat hippocampus are investigated in culture using a voltage‐sensitive fluorescent dye. The electrical response to somatic stimulation is observed as a time‐resolved map with a resolution of 0.9 μM at a time constant of 0.4 ms without signal averaging. The data are interpreted in terms of a tapering cable with Hodgkin‐Huxley parametrization. The spread of short hyperpolarizing transients is damped by capacitive shunting. The invasion of an action potential is boosted by voltage‐gated conductances of a low density. No irregularity is observed at a bifurcation. The passive cable parameters of internal resistance and membrane resistance at resting voltage are Ri= 300 ωcm and Rm= 40 kωcm2 respectively, at a maximum sodium conductance of ˜4.4 mS/cm2. The electrotonic length constant and the dynamic length constant at 1 kHz are 580 and 90 μm respectively. These results are compatible with electrophysiological data of dendrites in slices of adult hippocampus and with optical data of narrow processes of leech neurons in culture. The functional implications of boosting an action potential by voltage‐gated channels of low density are considered.
影响因子：
5.5
作者：
LLINAS, R;SUGIMORI, M
通讯作者：
SUGIMORI, M
合体中电紧张传导的 MSORTV 成像：简单唾液腺中偏振传播的光学记录。
DOI：
10.1002/jez.1402440110
发表时间：
1987
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
Senseman,DM;Horwitz,IS;Salzberg,BM
通讯作者：
Salzberg,BM