The nestin-expressing and non-expressing neurons in rat basal forebrain display different electrophysiological properties and project to hippocampus.
The nestin-expressing and non-expressing neurons in rat basal forebrain display different electrophysiological properties and project to hippocampus.
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大鼠基底前脑中表达巢蛋白和不表达巢蛋白的神经元表现出不同的电生理特性并投射到海马
DOI：
10.1186/1471-2202-12-129
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发表时间：
2011-12-20
期刊：
影响因子：
2.4
通讯作者：
Gao T
中科院分区：
文献类型：
--
作者：
Zhu J;Gu H;Yao Z;Zou J;Guo K;Li D;Gao T
背景在成年大鼠和人的内侧隔/斜角带复合体（medial septal/diagonal band complex，MS/DBB）中发现了Nestin免疫反应阳性（nestin-ir）神经元，但Nestin在功能神经元中的表达意义尚不清楚。本研究采用全细胞记录结合单细胞RT-PCR的方法，研究了nestin阳性神经元的电生理特性和神经化学表型，以探讨nestin在MS/DBB神经元中表达的意义。结果单细胞RT-PCR结果显示，87.5%（35/40）的nestin+细胞表达胆碱乙酰转移酶（ChAT+）mRNA，仅44.3%（35/79）的ChAT+细胞表达nestin mRNA。此外，没有巢蛋白+细胞表达谷氨酸脱羧酶67（GAD 67）或囊泡谷氨酸转运蛋白（VGLUT）mRNA。所有记录的nestin+细胞都是可兴奋的，并表现出慢放电特性，这与GAD 67或VGLUT mRNA阳性神经元的特性不同。结果表明，MS/DBB胆碱能神经元可分为表达nestin的胆碱能神经元（NEChs）和不表达nestin的胆碱能神经元（NNChs）。有趣的是，NEChs比NNChs具有更高的兴奋性，并接受更强的自发兴奋性突触输入。逆行标记结合胆碱乙酰转移酶和巢蛋白免疫荧光显示，无论是NEChs和NNChs的投射到hippocampa.ConclusionsThese结果表明，有两个平行的胆碱能隔-海马通路，可能有不同的功能。本文还对巢蛋白在功能神经元中表达的意义进行了讨论。
BackgroundNestin-immunoreactive (nestin-ir) neurons have been identified in the medial septal/diagonal band complex (MS/DBB) of adult rat and human, but the significance of nestin expression in functional neurons is not clear. This study investigated electrophysiological properties and neurochemical phenotypes of nestin-expressing (nestin+) neurons using whole-cell recording combined with single-cell RT-PCR to explore the significance of nestin expression in functional MS/DBB neurons. The retrograde labelling and immunofluorescence were used to investigate the nestin+ neuron related circuit in the septo-hippocampal pathway.ResultsThe results of single-cell RT-PCR showed that 87.5% (35/40) of nestin+ cells expressed choline acetyltransferase mRNA (ChAT+), only 44.3% (35/79) of ChAT+ cells expressed nestin mRNA. Furthermore, none of the nestin+ cells expressed glutamic acid decarboxylases 67 (GAD67) or vesicular glutamate transporters (VGLUT) mRNA. All of the recorded nestin+ cells were excitable and demonstrated slow-firing properties, which were distinctive from those of GAD67or VGLUT mRNA-positive neurons. These results show that the MS/DBB cholinergic neurons could be divided into nestin-expressing cholinergic neurons (NEChs) and nestin non-expressing cholinergic neurons (NNChs). Interestingly, NEChs had higher excitability and received stronger spontaneous excitatory synaptic inputs than NNChs. Retrograde labelling combined with choline acetyltransferase and nestin immunofluorescence showed that both of the NEChs and NNChs projected to hippocampus.ConclusionsThese results suggest that there are two parallel cholinergic septo-hippocampal pathways that may have different functions. The significance of nestin expressing in functional neurons has been discussed.
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影响因子：
2.8
作者：
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通讯作者：
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