Probing of the nuclear import and export signals and subcellular transport mechanism of varicella-zoster virus tegument protein open reading frame 10
Probing of the nuclear import and export signals and subcellular transport mechanism of varicella-zoster virus tegument protein open reading frame 10
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水痘带状疱疹病毒外皮蛋白开放阅读框10的核进出口信号及亚细胞转运机制探讨
DOI：
10.1007/s00430-011-0211-4
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发表时间：
2012-02
影响因子：
5.4
通讯作者：
Zheng, Chunfu
中科院分区：
文献类型：
--
作者：
Cai, Mingsheng;Wang, Shuai;Long, Jing;Zheng, Chunfu
水痘-带状疱疹病毒开放阅读框架10(ORF10)是病毒粒子中存在的一种被膜蛋白，是甲型疱疹病毒UL48基因家族的成员，与UL48原型单纯疱疹病毒1型VP16有相当大的氨基酸序列同源性。VP16具有多种功能，包括病毒即刻早期基因的转录激活。此外，VP16已被证明参与病毒组装和/或成熟的某些方面。然而，人们对ORF10的功能知之甚少。在这里，我们发现ORF10与增强的黄色荧光蛋白(EYFP)融合在COS-7细胞中的瞬时表达显示出在没有其他病毒蛋白的情况下以核为主的定位。通过构建一系列与EYFP融合的ORF10变异体，首次确定了ORF10的两段核定位信号，并将其定位到氨基酸(AA)302-347。此外，核输出信号(NES)被识别并发现在AA 226-236的亮氨酸富集区。最后证明，ORF10通过功能NES通过染色体区域维持1依赖的途径靶向细胞质，通过RAN和Importinβ1依赖的途径靶向细胞核，而不需要Importinα5。
Varicella-zoster virus open reading frame 10 (ORF10), a tegument protein present in the virion, is a member of the alphaherpesvirus UL48 gene family that shares considerable amino acid sequence homology with the UL48 prototype, herpes simplex virus type 1 VP16. VP16 serves multiple functions, including transcriptional activation of viral immediate-early genes. Furthermore, VP16 has been shown to be involved in some aspects of virus assembly and/or maturation. However, little is known concerning the function of ORF10. Here, we found that transient expression of ORF10 fused to enhanced yellow fluorescent protein (EYFP) in COS-7 cells showed the predominantly nuclear localization in the absence of other viral proteins. By constructing a series of ORF10 variants fused to EYFP, abona fidebipartite nuclear localization signal of ORF10 was, for the first time, determined and mapped to amino acids (aa) 302–347. Additionally, the nuclear export signal (NES) was identified and found to be in a leucine-rich region at aa 226–236. Finally, ORF10 was demonstrated to be targeted to the cytoplasm through the functional NES by chromosomal region maintenance 1-dependent pathway, and to the nucleus via Ran and importin β1-dependent pathway that does not require importin α5.
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影响因子：
64.5
作者：
Fornerod, M;Ohno, M;Mattaj, IW
通讯作者：
Mattaj, IW
影响因子：
7.7
作者：
Simpson, JC;Wellenreuther, R;Wiemann, S
通讯作者：
Wiemann, S
影响因子：
5.4
作者：
COHEN, JI;SEIDEL, K
通讯作者：
SEIDEL, K
影响因子：
5.4
作者：
Mossman, KL;Sherburne, R;Smiley, JR
通讯作者：
Smiley, JR
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
M. Cai;Shuai Wang;Junji Xing;C. Zheng
通讯作者：
M. Cai;Shuai Wang;Junji Xing;C. Zheng