Multiple <i>Colletotrichum</i> species commonly exhibit focal effector accumulation in a biotrophic interface at the primary invasion sites in their host plants
Multiple <i>Colletotrichum</i> species commonly exhibit focal effector accumulation in a biotrophic interface at the primary invasion sites in their host plants
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多个炭疽菌属物种通常在其寄主植物的主要入侵位点的生物营养界面中表现出焦点效应子积累
DOI：
10.1080/15592324.2021.1935604
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发表时间：
2021
影响因子：
2.9
通讯作者：
Takano Yoshitaka
中科院分区：
文献类型：
--
作者：
Ogawa Taiki;Chen Jinlian;Mise Kazuyuki;Takano Yoshitaka
真菌植物病原体会释放一套被称为效应器的分泌蛋白，以促进成功的感染。据报道，几种真菌病原体在宿主-病原菌的界面空间中分泌和积累其效应蛋白。此前，我们报道了南瓜炭疽病病原菌104-T菌株在附着体下方形成环形荧光信号的同时，产生并积累了带有mCherry标记的效应物。然而，目前还不清楚这些效应积累模式是否发生在其他C。属于炭疽菌属的轮状分离物和其他种类。在这里，我们研究了在宿主感染Fc过程中效应器的定位。OrbylareMAFF306589、三叶C.trifoliiMAFF305078，侵染紫花苜蓿ANDC。HigisianumMAFF305635，侵染十字花科植物。我们生成了每个物种的效应器-报告线，它们结构性地表达了mCherry标记的CoDN3效应器(CoDN3：mCherry)。免疫印迹分析检测到CoDN3：mCherry，证实有真菌细胞分泌CoDN3：mCherry。通过在相应寄主植物上的接种试验，我们在所有供试报告系的附着体侵染部位周围检测到环状CoDN3：mCherry荧光。这些结果表明，无论寄主不同，炭疽菌在进化上都保留了感染阶段分泌效应物的特性。
Fungal plant pathogens deploy a suite of secreted proteins, called effectors, to facilitate successful infection. Several fungal pathogens have been reported to secrete and accumulate their effector proteins in the host–pathogen interfacial spaces. Previously, we reported that the strain 104-T of the cucurbit anthracnose pathogenColletotrichum orbicularesecretes and accumulates mCherry-tagged effectors along with the formation of ring-shaped fluorescence signals beneath the appressoria. However, it was unclear whether these effector accumulation patterns occur in otherC. orbiculareisolates and other species belonging to theColletotrichumgenus. Here, we investigated the effector localization during host infection ofC. orbiculareMAFF306589,C. trifoliiMAFF305078, which infects alfalfa, andC. higginsianumMAFF305635, which infects Brassicaceae plants. We generated effector–reporter lines of each species, which constitutively expressed mCherry-tagged CoDN3 effector (CoDN3:mCherry). Immunoblotting analysis of the liquid culture fluids of the generated lines detected CoDN3:mCherry, which confirmed secretion of CoDN3:mCherry by fungal cells. Via inoculation assays in the corresponding host plants, we detected ring-shaped CoDN3:mCherry fluorescence around the appressorial invasion sites in all tested reporter lines. These results suggest that pathogens in theColletotrichumgenus have evolutionarily conserved the trait of effector secretion in the infection stage irrespective of differences in their hosts.
影响因子：
10.2
作者：
Toruño TY;Stergiopoulos I;Coaker G
通讯作者：
Coaker G