Differences of Matrix Metalloproteinase 2 Expression between Left and Right Ventricles in Response to Nandrolone Decanoate and/or Swimming Training in Mice.
Differences of Matrix Metalloproteinase 2 Expression between Left and Right Ventricles in Response to Nandrolone Decanoate and/or Swimming Training in Mice.
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小鼠左心室和右心室对癸酸诺龙和/或游泳训练反应中基质金属蛋白酶 2 表达的差异
DOI：
10.4103/0366-6999.222330
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发表时间：
2018-01-20
影响因子：
6.1
通讯作者：
Esteve-Pastor MA
中科院分区：
文献类型：
--
作者：
Bai Y;Shi XB;Zhang YQ;Wang YL;Liu XY;Esteve-Pastor MA
背景：基质金属蛋白酶（Matrix metalloproteinase， MMP）-2在左心室（LVs）和右心室（RVs）重构中起重要作用。我们基于MMP-2的结构、功能和生化特征，研究了小鼠左心室和右心室在壬酸诺龙（ND）、游泳训练（ST）和ND + ST联合（NS）作用下MMP-2表达的差异。方法：雄性C57B1小鼠28只（6周龄，20 ~ 23 g），随机分为对照组（n = 7）、ND组（n = 6）、ST组（n = 8）、NS组（n = 7）。分别治疗8周后，采用超声心动图检查心脏结构和功能。采用Van Gieson染色法检测不同治疗后左室和右室纤维化情况，采用Western blotting分析分析左室和右室对ND和/或st的MMP-2表达差异。结果：8周时，超声心动图显示ND组右心室尺寸/体重大于其他3组（F = 7.12, P < 0.05）。ND诱导的纤维化在右心室（2.59%）高于左心室（2.21%）。ND组RV中MMP-2的表达显著高于对照组和NS组RV及相应ND组LV。结论：实验数据支持ND诱导右心室MMP-2表达高于左心室的假设，从而导致右心室扩张。
Background: Matrix metalloproteinase (MMP)-2 plays an important role in the remodeling of left ventricles (LVs) and right ventricles (RVs). We investigated the differences of MMP-2 expression between LV and RV in response to nandrolone decanoate (ND), swimming training (ST), and combined ND and ST (NS) in mice, based on their structural, functional, and biochemical characteristics. Methods: Totally 28 male C57B1 mice (6 weeks old; 20–23 g) were divided into four groups, including the control (n = 7), ND (n = 6), ST (n = 8), and NS (n = 7) groups. After respective treatments for 8 weeks, echocardiographic examination was used to assess the cardiac structure and function. Van Gieson stain was used to examine the fibrosis of LV and RV in response to different treatments, and Western blotting analysis was performed to explore different MMP-2 expressions between LV and RV in response to ND and/or ST. Analysis of variance was used for comparing the four groups. Results: At 8 weeks, right ventricular dimension/body weight in the ND group was larger than the other three groups (F = 7.12, P < 0.05) according to the echocardiographic examination. Fibrosis induced by ND administration was increased more in RV (2.59%) than that in LV (2.21%). MMP-2 expression of the ND group in RV was significantly greater than the control and NS groups in RV and the corresponding ND group in LV. Conclusion: The experimental data support the hypothesis that ND administration induces greater MMP-2 expression increase in RV compared to LV, leading to consequent RV dilation.
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影响因子：
37.8
作者：
Benito, Begona;Gay-Jordi, Gemma;Mont, Lluis
通讯作者：
Mont, Lluis
影响因子：
4.8
作者：
BAUMAN, DH;RICHERSON, JT;BRITT, AL
通讯作者：
BRITT, AL
影响因子：
3.7
作者：
Bocalini DS;Beutel A;Bergamaschi CT;Tucci PJ;Campos RR
通讯作者：
Campos RR
DOI：
10.1152/ajpheart.00831.2007
发表时间：
2008-04-01
影响因子：
4.8
作者：
Yang, Yining;Ma, Yitong;Gao, Xiao-Ming
通讯作者：
Gao, Xiao-Ming
DOI：
10.3791/50912
发表时间：
2013-11-27
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Brittain E;Penner NL;West J;Hemnes A
通讯作者：
Hemnes A