RECOMBINANT BOVINE HERPESVIRUS-1 EXPRESSING p23 PROTEIN OF CRYPTOSPORIDIUM PARVUM INDUCES NEUTRALIZING ANTIBODIES IN RABBITS
RECOMBINANT BOVINE HERPESVIRUS-1 EXPRESSING p23 PROTEIN OF CRYPTOSPORIDIUM PARVUM INDUCES NEUTRALIZING ANTIBODIES IN RABBITS
复制标题
表达小隐孢子虫 p23 蛋白的重组牛疱疹病毒-1 在兔中诱导中和抗体
作者：
Y. Takashima;X. Xuan;I. Kimata;M. Iseki;Y. Kodama;Noriko Nagane;H. Nagasawa;Y. Matsumoto;T. Mikami;H. Otsuka
为了研制抗牛隐孢子虫病的疫苗，我们构建了表达微小隐孢子虫子孢子表面蛋白p23的重组牛疱疹病毒1型（BHV-1）。在重组病毒中，CAG启动子控制下的p23基因和编码增强型绿色荧光蛋白的基因被整合到BHV-1的gG基因中。尽管同源重组的频率较低，但由于重组体形成的噬菌斑具有特异性荧光，因此重组体的克隆很容易。这些空斑是非荧光亲本病毒的空斑之一。选择用于荧光的所有克隆也含有p23基因。在用重组BHV-1感染的MDBK细胞中，抗p23蛋白的抗体在Western印迹分析中识别p23蛋白为约23-kDa的特异性条带。用重组蛋白免疫家兔产生抗p23蛋白的IgG。免疫兔血清可降低C. HCT-8细胞中的微小子孢子。与正常兔血清对照相比，免疫兔的血清减少了感染。这些结果表明，重组BHV-1在兔中诱导中和抗体。
In order to develop a vaccine against cryptosporidiosis in cattle, we constructed a recombinant bovine herpesvirus-1 (BHV-1) expressing an immunodominant surface protein, p23, of Cryptosporidium parvum sporozoites. In the recombinant virus, the p23 gene under the control of a CAG promoter and a gene coding for an enhanced green fluorescent protein were integrated into the gG gene of BHV-1. Despite a low frequency of homologous recombination, cloning of the recombinants was easy because of the specific fluorescence of the plaques formed by recombinants. These plaques were among the plaques of the nonfluorescent parental virus. All clones selected for fluorescence also contained the p23 gene. In MDBK cells infected with the recombinant BHV-1, the antibody against the p23 protein recognized the p23 protein as an approximately 23-kDa specific band in Western blotting analysis. Rabbits immunized with the recombinant produced IgG against the p23 protein. It was also demonstrated that the sera of immunized rabbits reduced infection of C. parvum sporozoites in HCT-8 cells. The serum of an immunized rabbit reduced infection compared with the normal rabbit serum control. These results indicate that the recombinant BHV-1 induces neutralizing antibodies in rabbits.
中和敏感表位暴露在传染性隐孢子虫子孢子的表面。
DOI：
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发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Riggs,MW;McGuire,TC;Mason,PH;Perryman,LE
通讯作者：
Perryman,LE
影响因子：
5.5
作者：
Perryman, LE;Kapil, SJ;Hunt, EL
通讯作者：
Hunt, EL