Establishing a Special Open Field Test Appliance for Tree Shrews Evaluates their Stressed Locomotor Behavior
Establishing a Special Open Field Test Appliance for Tree Shrews Evaluates their Stressed Locomotor Behavior
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为树鼩建立特殊的开放场地测试装置，评估其应激运动行为
DOI：
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Jun Gao
中科院分区：
文献类型：
--
作者：
Chuan He;Jing Dai;Minjie Zhou;Meng Lv;Jing Wang;Jun Gao
建立一套重复性好、专门性强的系统来评价树鼠动物模型，尤其是神经精神病学模型的运动能力，具有重要意义。为此，我们利用聚甲基丙烯酸甲酯板(有机玻璃)设计了一种专门的树鼠野外测试用具。在其中检测到了两次幼稚的树鼠的活动。通过使用任意迷宫软件跟踪运动轨迹，我们发现两个测试中的运动轨迹和距离非常相似。结果表明，为树鼠设计的器械具有极高的可靠性和稳定性。然后利用该系统对应激树鼠的活动进行评估，并观察到光应激2周后树鼠的活动减少。与此相一致的是，应激树鼠尿液中ACTH水平升高。综上所述，光胁迫可以显著减少小鼠的活动，这可以通过我们的开放式实验系统进行研究。此外，我们的专门的野外实验系统的数据稳定和准确，用于测试树鼠的运动行为。
It has a great significance to establish a high repetitive and special system to evaluate locomotion for tree shrew animal models, especially neuropsychiatric models. Thus, we designed a special open field test appliance for tree shrews by using a polymer PMMA board (plexiglass). The locomotion of naïve tree shrews was detected twice in it. By using Any-maze Software tracing the motion trajectory, we found that the motion trajectory and the distance were very similar in both tests. It demonstrated that the appliance designed for tree shrews had extremely high reliability and stability. Then we used this system to evaluate the activity of the stressed tree shrews, and observed the reduced activity of the tree shrews after a 2-week light-stress. Consistent with this, the ACTH level in the urine of the stressed tree shrews was enhanced. Taken together, the light stress could lead to a significant reduction of locomotion which could be investigated by our open field experiment system. Furthermore, the data from our special open field experiment system is stable and accurate for testing the locomotion behavior of tree shrews.
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DOI：
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发表时间：
1979-03
期刊：
American journal of mental deficiency
影响因子：
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作者：
R. Berk
通讯作者：
R. Berk
DOI：
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发表时间：
2016
期刊：
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影响因子：
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作者：
A. Foerster
通讯作者：
A. Foerster
影响因子：
2.7
作者：
Mineur, Yann S.;Belzung, Catherine;Crusio, Wim E.
通讯作者：
Crusio, Wim E.
DOI：
10.1523/jneurosci.16-10-03534.1996
发表时间：
1996-05
期刊：
The Journal of Neuroscience
影响因子：
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作者：
A. Magariños;B. McEwen;G. Flügge;E. Fuchs
通讯作者：
A. Magariños;B. McEwen;G. Flügge;E. Fuchs
DOI：
10.1073/pnas.0703615105
发表时间：
2008-03-25
影响因子：
11.1
作者：
Gonzalez, M. M. C.;Aston-Jones, G.
通讯作者：
Aston-Jones, G.