Measuring in vivo tumor pHe with CEST-FISP MRI.
Measuring in vivo tumor pHe with CEST-FISP MRI.
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DOI：
10.1002/mrm.23038
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发表时间：
2012-03
影响因子：
3.3
通讯作者：
Pagel, Mark D.
中科院分区：
文献类型：
--
作者：
Sheth, Vipul R.;Li, Yuguo;Chen, Liu Qi;Howison, Christine M.;Flask, Chris A.;Pagel, Mark D.
PARACEST MRI造影剂已经开发出来，可以在溶液研究中测量pH值，但这些造影剂以前没有在体内检测到。为了使用PARACEST试剂Yb-DO3A-oAA测量肿瘤组织的细胞外pH (pHe)，建立了CEST- fisp MRI方案，优化饱和周期以实现CEST的敏感检测，并采用中值滤波去除CEST光谱中的伪影。这些改进将pH与Yb-DO3A-oAA在7T磁场强度下的两种CEST效应的比值相关联（R2 = 0.99，精度标准偏差= 0.011 pH单位）。由于体内CEST MRI灵敏度较低，静脉注射后无法在肿瘤组织中检测到PARACEST药物。PARACEST药物直接注射到肿瘤组织和腿部肌肉中，检测到Yb-DO3A-oAA。测量的CEST效应用于测量肿瘤pH值（6.82±0.21）和腿部肌肉pH值（7.26±0.14），并从这些组织区域生成参数pH图。这些结果表明，肿瘤pHe可以用PARACEST试剂和快速CEST-MRI方案来测量。
PARACEST MRI contrast agents have been developed that can measure pH in solution studies, but these agents have not previously been detected in vivo. In order to use the PARACEST agent Yb-DO3A-oAA to measure the extracellular pH (pHe) in tumor tissue, a CEST-FISP MRI protocol was developed, the saturation period was optimized for sensitive CEST detection, and median filtering was used to remove artifacts in CEST spectra. These improvements were used to correlate pH with a ratio of two CEST effects of Yb-DO3A-oAA at a 7T magnetic field strength (R2 = 0.99, standard deviation of precision = 0.011 pH units). The PARACEST agent could not be detected in tumor tissue following i.v. injection due to the low sensitivity of in vivo CEST MRI. Yb-DO3A-oAA was detected in tumor tissue and leg muscle after directly injecting the PARACEST agent into these tissues. The measured CEST effects were used to measure a tumor pH of 6.82 ± 0.21 and a leg muscle pH of 7.26 ± 0.14, and parametric pH maps were also generated from these tissue regions. These results demonstrated that tumor pHe can be measured with a PARACEST agent and a rapid CEST-MRI protocol.
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CEST-FISP：一种在7 t处的快速化学交换饱和转移MRI的新技术。
影响因子：
3.3
作者：
Shah, T.;Lu, L.;Dell, K. M.;Pagel, M. D.;Griswold, M. A.;Flask, C. A.
通讯作者：
Flask, C. A.
影响因子：
3.3
作者：
Liu, Guanshu;Li, Yuguo;Pagel, Mark D.
通讯作者：
Pagel, Mark D.
影响因子：
10.6
作者：
Chan, RH;Ho, CW;Nikolova, M
通讯作者：
Nikolova, M
影响因子：
6.7
作者：
Parmelee, DJ;Walovitch, RC;Lauffer, RB
通讯作者：
Lauffer, RB
影响因子：
3.3
作者：
Kim M;Gillen J;Landman BA;Zhou J;van Zijl PC
通讯作者：
van Zijl PC