Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
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家蚕翅盘变态发育特征的转录组分析
DOI：
10.1186/1471-2164-15-820
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发表时间：
2014-09-27
期刊：
影响因子：
4.4
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Ou J;Deng HM;Zheng SC;Huang LH;Feng QL;Liu L
背景：B的翅盘。家蚕在幼虫到蛹的变态过程中转化为蛹翅，具有显著的形态和结构变化。为了从转录水平上了解这些变化，我们对6日龄5龄幼虫（L5D6）、前蛹（PP）和蛹（P0）的翅盘进行了RNA测序。RNA-seq数据综合分析结果表明，在幼虫至蛹变态过程中，20E信号通路的多个基因表达上调，JH信号通路的多个基因表达下调。17个转录因子表达显著上调。角质层蛋白基因（尤其是翅角质层蛋白基因）在P0期表达量最高，且表达量显著上调。负责几丁质降解和从头合成的基因显著上调。B中有A、B两种几丁质酶。而只有几丁质合成酶A表达上调。在L5D6、PP和P0的血淋巴中检测到几丁质合成的前体海藻糖和D-果糖，表明几丁质的从头合成。结论：获得了家蚕变态期翅盘发育过程中大量的转录组和DGE图谱数据，在转录水平上提供了较全面的基因表达信息。这些结果表明，在幼虫到蛹的变态过程中，蛹翅的发育和转变可能主要由B中的20 E信号控制。森。这17个上调的转录因子可能与翅发育有关。蛹翅发育所需的几丁质可能是由组分几丁质降解和从头合成两种途径产生的。几丁质合成酶A可能参与蛹翅中几丁质的合成，而海藻糖和D-果糖可能参与蛹翅形成过程中几丁质的从头合成。
Background:Wing discs of B. mori are transformed to pupal wings during the larva-to-pupa metamorphosis with dramatic morphological and structural changes. To understand these changes at a transcriptional level, RNA-seq of the wing discs from 6-day-old fifth instar larvae (L5D6), prepupae (PP) and pupae (P0) was performed.Results:In total, 12,254 transcripts were obtained from the wing disc, out of which 5,287 were identified to be differentially expressed from L5D6 to PP and from PP to P0. The results of comprehensive analysis of RNA-seq data showed that during larvae-to-pupae metamorphosis, many genes of 20E signaling pathway were up-regulated and those of JH signaling pathway were down-regulated. Seventeen transcription factors were significantly up-regulated. Cuticle protein genes (especially wing cuticle protein genes), were most abundant and significantly up-regulated at P0 stage. Genes responsible for the degradation and de novo synthesis of chitin were significantly up-regulated. There were A and B two types of chitin synthases in B. mori, whereas only chitin synthase A was up-regulated. Both trehalose and D-fructose, which are precursors of chitin synthesis, were detected in the hemolymph of L5D6, PP and P0, suggesting de novo synthesis of chitin. However, most of the genes that are related to early wing disc differentiation were down-regulated.Conclusions:Extensive transcriptome and DGE profiling data of wing disc during metamorphosis of silkworm have been generated, which provided comprehensive gene expression information at the transcriptional level. These results implied that during the larva-to-pupa metamorphosis, pupal wing development and transition might be mainly controlled by 20E signaling in B. mori. The 17 up-regulated transcription factors might be involved in wing development. Chitin required for pupal wing development might be generated from both degradation of componential chitin and de novo synthesis. Chitin synthase A might be responsible for the chitin synthesis in the pupal wing, while both trehalose and D-fructose might contribute to the de novo synthesis of chitin during the formation of pupal wing.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
10.5
作者：
Bergsland, Maria;Werme, Martin;Muhr, Jonas
通讯作者：
Muhr, Jonas
DOI：
10.1073/pnas.1116123109
发表时间：
2011-12-27
影响因子：
11.1
作者：
Charles, Jean-Philippe;Iwema, Thomas;Jindra, Marek
通讯作者：
Jindra, Marek
影响因子：
3.5
作者：
Ali, Md Saheb;Iwanaga, Masashi;Kawasaki, Hideki
通讯作者：
Kawasaki, Hideki
DOI：
10.1673/031.010.11401
发表时间：
2010
期刊：
Journal of insect science (Online)
影响因子：
--
作者：
Gan LP;Zhang WY;Niu YS;Xu L;Xi J;Ji MM;Xu SQ
通讯作者：
Xu SQ