Targeted imaging of esophageal adenocarcinoma with a near-infrared fluorescent peptide.
Targeted imaging of esophageal adenocarcinoma with a near-infrared fluorescent peptide.
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DOI：
10.1186/s12876-021-01840-3
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发表时间：
2021-06-12
影响因子：
2.4
通讯作者：
Wu K
中科院分区：
文献类型：
--
作者：
Kang X;Li M;Liu L;Liu S;Hu H;Zhang R;Ning S;Tian Z;Pan Y;Guo X;Wu K
靶向光学成像为胃肠道肿瘤的早期检测提供了一种无创和准确的方法，特别是对于扁平外观。在我们前期的研究中，我们利用噬菌体展示技术鉴定了一段SNFYMPL（SNF）序列作为食管癌特异性结合肽。本研究旨在评价Cy5.5标记SNF探针在体内外食管癌显像中的靶向性。合成SNF-Cy 5.5探针，并通过高效液相色谱（HPLC）和质谱（MS）进行鉴定。进行共聚焦荧光成像和流式细胞术分析以评估SNF-Cy 5.5与OE 33的结合特异性和受体结合亲和力。体内显像评价SNF-Cy 5.5对食管癌的靶向性。共聚焦成像和流式细胞仪分析显示SNF-Cy 5.5与OE 33细胞质膜特异性结合，并具有较高的亲和力。在体内，对于非阻断组，SNF-Cy 5.5探针在24 h p.i.并且在2 h p.i.对于阻断组，在4小时后在小鼠中未观察到SNF-Cy 5.5。离体显像显示非阻断组肿瘤荧光信号强度明显高于阻断组（2.6 ± 0.32 × 109 vs. 0.8 ± 0.08 × 109，p < 0.05）。合成了SNF-Cy 5.5，并对其进行了表征。SNF-Cy 5.5具有较高的亲和性、特异性和肿瘤靶向性。SNF-Cy 5.5是一种很有前途的用于食管腺癌成像的光学探针。在线版本包含补充材料，可通过10.1186/s12876-021-01840-3获得。
Targeted optical imaging offers a noninvasive and accurate method for the early detection of gastrointestinal tumors, especially for flat appearances. In our previous study, a sequence of SNFYMPL (SNF) was identified as a specific peptide to bind to esophageal carcinoma using phage-display technology. This study aimed to evaluate the tumor-targeting efficacy of Cy5.5-conjugated SNF probe for imaging of esophageal carcinoma in vitro and in vivo. The SNF-Cy5.5 probe was synthesized and then identified using High Performance Liquid Chromatography (HPLC) and mass spectrometry (MS). Confocal fluorescence imaging and Flow cytometry analysis were performed to evaluate the binding specificity and the receptor binding affinity of SNF-Cy5.5 to OE33. In vivo imaging was performed to evaluate the targeting ability of SNF-Cy5.5 to esophageal carcinoma. The confocal imaging and flow cytometry analysis showed that SNF-Cy5.5 bound specifically to the plasma membrane of OE33 cells with a high affinity. In vivo, for non-block group, SNF-Cy5.5 probe exhibited rapid OE33 tumor targeting during 24 h p.i. and excellent tumor-to-background contrast at 2 h p.i. For the block group, SNF-Cy5.5 was not observed in the mice after 4 h p.i. Ex vivo imaging also revealed that a higher fluorescent signal intensity value of the tumors was clearly observed in the non-block group than that in the block group (2.6 ± 0.32 × 109 vs. 0.8 ± 0.08 × 109, p < 0.05). SNF-Cy5.5 was synthesized and characterized with a high efficiency and purity. The higher affinity, specificity, and tumor targeting efficacy of SNF-Cy5.5 were confirmed by in vitro and in vivo tests. SNF-Cy5.5 is a promising optical probe for the imaging of esophageal adenocarcinoma. The online version contains supplementary material available at 10.1186/s12876-021-01840-3.
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影响因子：
5.8
作者：
Feng, Shuo;Zhou, Juan;Wang, Thomas D.
通讯作者：
Wang, Thomas D.
影响因子：
7.3
作者：
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通讯作者：
Wang TD
影响因子：
2.8
作者：
Ding S;Blue RE;Moorefield E;Yuan H;Lund PK
通讯作者：
Lund PK
影响因子：
29.4
作者：
Li M;Anastassiades CP;Joshi B;Komarck CM;Piraka C;Elmunzer BJ;Turgeon DK;Johnson TD;Appelman H;Beer DG;Wang TD
通讯作者：
Wang TD
DOI：
10.1007/s00259-011-1886-x
发表时间：
2011-11
影响因子：
9.1
作者：
Yang, Yunan;Zhang, Yin;Hong, Hao;Liu, Glenn;Leigh, Bryan R.;Cai, Weibo
通讯作者：
Cai, Weibo