Ex Vivo and In Vivo Noninvasive Imaging of Epidermal Growth Factor Receptor Inhibition on Colon Tumorigenesis Using Activatable Near-Infrared Fluorescent Probes.
Ex Vivo and In Vivo Noninvasive Imaging of Epidermal Growth Factor Receptor Inhibition on Colon Tumorigenesis Using Activatable Near-Infrared Fluorescent Probes.
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DOI：
10.1177/1536012117729044
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发表时间：
2017-01
影响因子：
2.8
通讯作者：
Lund PK
中科院分区：
文献类型：
--
作者：
Ding S;Blue RE;Moorefield E;Yuan H;Lund PK
近红外荧光（NIRF）成像结合酶激活探针在癌症检测中取得了很好的结果。检测三维（3-D）无创伤体内NIRF成像能否检测表皮生长因子受体（EGFR）抑制剂对偶氮氧甲烷（AOM）诱导的结肠肿瘤或Apc Min/+小鼠肿瘤息肉样和扁平肿瘤负荷的影响。aom注射的KK-HIJ小鼠分别饲喂EGFR抑制剂饲粮或鼠粮。这些小鼠和Apc Min/+小鼠在成像前给予组织蛋白酶激活探针（ProSense 680）。体内成像采用定量层析NIRF成像。进行了离体成像和组织学检查。采用微型计算机断层扫描（CT）和三维NIRF成像双重成像来确定肿瘤的位置。体外使用组织蛋白酶B探针检测EGFR抑制对肿瘤负荷的降低。体内成像显示探针仅在大肿瘤中有强烈的激活。microCT和3D NIRF双重成像提高了体内肿瘤的检测。ProSense 680三维NIRF成像技术可以在体外检测和量化药物对结肠肿瘤的影响。ProSense 680探针的NIRF成像作为一种有效的非内镜肠道肿瘤检测方法存在局限性。与其他影像学手段相结合，可提高体内肠道肿瘤检测的特异性和敏感性。
Near-infrared fluorescence (NIRF) imaging combined with enzyme-activatable NIRF probes has yielded promising results in cancer detection. To test whether 3-dimensional (3-D) noninvasive in vivo NIRF imaging can detect effects of epidermal growth factor receptor (EGFR) inhibitor on both polypoid and flat tumor load in azoxymethane (AOM)-induced colon tumors or tumors in Apc Min/+ mice. The AOM-injected KK-HIJ mice received EGFR inhibitor diet or chow diet. These and Apc Min/+ mice were given cathepsin-activatable probes (ProSense 680) before imaging. In vivo imaging was performed using quantitative tomographic NIRF imaging. Ex vivo imaging and histologic examination were performed. Dual imaging by micro computed tomography (CT) and 3D NIRF imaging was used to verify tumor location. Tumor load reduction by EGFR inhibition was detected ex vivo using cathepsin B probes. In vivo imaging revealed intense activation of probes only in large tumors. Dual imaging with microCT and 3D NIRF imaging improved tumor detection in vivo. The 3-D NIRF imaging with ProSense 680 can detect and quantify drug effects on colon tumors ex vivo. The NIRF imaging with ProSense 680 probe has limitations as a valid nonendoscopic method for intestinal tumor detection. Combing with other imaging modalities will improve the specificity and sensitivity of intestinal tumor detection in vivo.
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影响因子：
62.1
作者：
Louie, Angelique Y.
通讯作者：
Louie, Angelique Y.
影响因子：
17.1
作者：
Kunjachan S;Gremse F;Theek B;Koczera P;Pola R;Pechar M;Etrych T;Ulbrich K;Storm G;Kiessling F;Lammers T
通讯作者：
Lammers T
影响因子：
9.3
作者：
Heresbach, D.;Barrioz, T.;Ponchan, T.
通讯作者：
Ponchan, T.
影响因子：
12.4
作者：
Habibollahi P;Figueiredo JL;Heidari P;Dulak AM;Imamura Y;Bass AJ;Ogino S;Chan AT;Mahmood U
通讯作者：
Mahmood U
影响因子：
56.9
作者：
MOSER, AR;PITOT, HC;DOVE, WF
通讯作者：
DOVE, WF