Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts.
Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts.
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DOI：
10.1016/j.celrep.2017.05.019
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发表时间：
2017-05-30
期刊：
影响因子：
8.8
通讯作者：
Slukvin II
中科院分区：
文献类型：
--
作者：
Kumar A;D'Souza SS;Moskvin OV;Toh H;Wang B;Zhang J;Swanson S;Guo LW;Thomson JA;Slukvin II
阐明通向血管生成细胞的途径，并能够鉴定其祖细胞和谱系受限细胞，对于建立人类多能干细胞(HPSC)血管疾病模型和开发血管疗法至关重要。在这里，我们发现中胚层来源的周细胞(PC)和平滑肌细胞(SMC)起源于克隆的间充质祖细胞-间充质血管母细胞(MB)。在克隆性培养中，骨髓间充质干细胞分化为原始的PDGFRβ+CD271+CD73−间充质前体细胞，从而产生增殖的PC、SMC和间充质干细胞/基质细胞。MB来源的PC可进一步定位为CD274+毛细血管PC和DLK1+小动脉PC，分别具有促炎和收缩表型。用MEK抑制剂诱导SMC成熟。建立血管生成谱系树，以及鉴定阶段和谱系特异性标记，为询问调节血管生成细胞规格和多样化的分子机制提供了平台，并为从hPSCs制造用于血管工程和细胞治疗的明确的壁细胞群提供了平台。Kumar等人研究发现，在人类多能干细胞培养中，中胚层周细胞和平滑肌细胞来自一种常见的内皮细胞和间充质细胞前体--间充质血管母细胞。他们展示了不同的壁细胞谱系是如何从间充质血管母细胞中特定出来的，并定义了血管生成细胞的阶段和谱系特异性标记物。
Elucidating the pathways that lead to vasculogenic cells, and being able to identify their progenitors and lineage-restricted cells, is critical to the establishment of human pluripotent stem cell (hPSC) models for vascular diseases and development of vascular therapies. Here, we find that mesoderm-derived pericytes (PCs) and smooth muscle cells (SMCs) originate from a clonal mesenchymal progenitor mesenchymoangioblast (MB). In clonogenic cultures, MBs differentiate into primitive PDGFRβ+ CD271+CD73− mesenchymal progenitors, which give rise to proliferative PCs, SMCs, and mesenchymal stem/stromal cells. MB-derived PCs can be further specified to CD274+ capillary and DLK1+ arteriolar PCs with a proinflammatory and contractile phenotype, respectively. SMC maturation was induced using a MEK inhibitor. Establishing the vasculogenic lineage tree, along with identification of stage- and lineage-specific markers, provides a platform for interrogating the molecular mechanisms that regulate vasculogenic cell specification and diversification and manufacturing well-defined mural cell populations for vascular engineering and cellular therapies from hPSCs. Kumar et al. find that mesodermal pericytes and smooth muscle cells in human pluripotent stem cell cultures originate from a common endothelial and mesenchymal cell precursor, the mesenchymoangioblast. They show how different lineages of mural cells are specified from mesenchymoangioblasts and define stage- and lineage-specific markers for vasculogenic cells.
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DOI：
10.1126/science.aad0084
发表时间：
2016-01-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Khan JA;Mendelson A;Kunisaki Y;Birbrair A;Kou Y;Arnal-Estapé A;Pinho S;Ciero P;Nakahara F;Ma'ayan A;Bergman A;Merad M;Frenette PS
通讯作者：
Frenette PS
影响因子：
2.5
作者：
Maeda, J;Yamagishi, H;Srivastava, D
通讯作者：
Srivastava, D
影响因子：
6
作者：
Chambers, RC;Leoni, P;Heller, RA
通讯作者：
Heller, RA
影响因子：
10.8
作者：
Bajpai, Vivek K.;Mistriotis, Panagiotis;Andreadis, Stelios T.
通讯作者：
Andreadis, Stelios T.
DOI：
10.1111/j.1749-6632.2009.04967.x
发表时间：
2009-01-01
期刊：
HEMATOPOIETIC STEM CELLS VII
影响因子：
--
作者：
Crisan, Mihaela;Chen, Chien-Wen;Peault, Bruno
通讯作者：
Peault, Bruno