Target site specificity and in vivo complexity of the mammalian arginylome.
Target site specificity and in vivo complexity of the mammalian arginylome.
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DOI：
10.1038/s41598-018-34639-6
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发表时间：
2018-11-01
影响因子：
4.6
通讯作者：
Kashina A
中科院分区：
文献类型：
--
作者：
Wang J;Pejaver VR;Dann GP;Wolf MY;Kellis M;Huang Y;Garcia BA;Radivojac P;Kashina A
Protein arginylation mediated by arginyltransferase ATE1 is a key regulatory process essential for mammalian embryogenesis, cell migration, and protein regulation. Despite decades of studies, very little is known about the specificity of ATE1-mediated target site recognition. Here, we used in vitro assays and computational analysis to dissect target site specificity of mouse arginyltransferases and gain insights into the complexity of the mammalian arginylome. We found that the four ATE1 isoforms have different, only partially overlapping target site specificity that includes more variability in the target residues than previously believed. Based on all the available data, we generated an algorithm for identifying potential arginylation consensus motif and used this algorithm for global prediction of proteins arginylated in vivo on the N-terminal D and E. Our analysis reveals multiple proteins with potential ATE1 target sites and expand our understanding of the biological complexity of the intracellular arginylome.
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影响因子：
2.7
作者：
Saha S;Kashina A
通讯作者：
Kashina A
影响因子：
--
作者：
Saha S;Wong CC;Xu T;Namgoong S;Zebroski H;Yates JR 3rd;Kashina A
通讯作者：
Kashina A
影响因子：
5
作者：
Kurosaka, Satoshi;Leu, N. Adrian;Kashina, Anna
通讯作者：
Kashina, Anna
DOI：
10.1093/bioinformatics/btr209
发表时间：
2011-07-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Lin MF;Jungreis I;Kellis M
通讯作者：
Kellis M
影响因子：
4
作者：
Khanal, Neelam;Pejaver, Vikas;Mukhopadhyay, Suchetana
通讯作者：
Mukhopadhyay, Suchetana