Mitochondrial DNA (mtDNA) haplogroups and serum levels of anti-oxidant enzymes in patients with osteoarthritis.
Mitochondrial DNA (mtDNA) haplogroups and serum levels of anti-oxidant enzymes in patients with osteoarthritis.
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DOI：
10.1186/1471-2474-12-264
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发表时间：
2011-11-22
影响因子：
2.3
通讯作者：
Blanco FJ
中科院分区：
文献类型：
--
作者：
Fernandez-Moreno M;Soto-Hermida A;Pertega S;Oreiro N;Fernandez-Lopez C;Rego-Perez I;Blanco FJ
氧化应激在骨性关节炎的发生和发展中起主要作用，并导致线粒体退变。为了防止这种情况，软骨细胞拥有一个协调良好的酶促抗氧化系统。此外，线粒体DNA(MtDNA)单倍群与骨性关节炎相关。因此，这项工作的主要目的是评估mtDNA单倍群对血清中两种主要抗氧化酶--锰超氧化物歧化酶(Mn-SOD或SOD2)和过氧化氢酶水平的影响，并测试这两种蛋白作为潜在的OA相关生物标志物的适用性。采用双抗体夹心ELISA法对73例骨性关节炎患者和77例携带J、U、H单倍型的健康人血清SOD2和过氧化氢酶水平进行了分析。按Kellgren和Lawrence(K/L)0~IV级标准对膝关节和髋关节X线片进行分级，并对性别、体重指数、年龄、吸烟、诊断、单倍型和放射学K/L分级等临床变量对血清上述酶水平的影响进行统计学分析。与健康对照组相比，骨性关节炎患者血清SOD2水平明显升高(P<；0.001)。即使在严重程度较高的骨性关节炎患者(K/L分级IV级)中，血清中这种抗氧化酶的水平也明显高于K/L分级较低的患者(p<；0.001)。线粒体DNA单倍型对血清过氧化氢酶水平有影响(p=0.054)，J单倍型携带者血清过氧化氢酶水平高于非J携带者(p=0.057)。骨性关节炎患者体内SOD2水平升高提示与骨性关节炎相关的氧化应激增加，因此这种抗氧化酶可能是诊断骨性关节炎的一个合适的候选生物标志物。线粒体单倍群与血清过氧化氢酶水平显著相关
Oxidative stress play a main role in the initiation and progression of the OA disease and leads to the degeneration of mitochondria. To prevent this, the chondrocytes possess a well-coordinated enzymatic antioxidant system. Besides, the mitochondrial DNA (mtDNA) haplogroups are associated with the OA disease. Thus, the main goal of this work is to assess the incidence of the mtDNA haplogroups on serum levels of two of the main antioxidant enzymes, Manganese Superoxide Dismutase (Mn-SOD or SOD2) and catalase, and to test the suitability of these two proteins for potential OA-related biomarkers. We analyzed the serum levels of SOD2 and catalase in 73 OA patients and 77 healthy controls carrying the haplogroups J, U and H, by ELISA assay. Knee and hip radiographs were classified according to Kellgren and Lawrence (K/L) scoring from Grade 0 to Grade IV. Appropriate statistical analyses were performed to test the effects of clinical variables, including gender, body mass index (BMI), age, smoking status, diagnosis, haplogroups and radiologic K/L grade on serum levels of these enzymes. Serum levels of SOD2 appeared statistically increased in OA patients when compared with healthy controls (p < 0.001). Even in those OA patients with higher OA severity (K/L grade IV), the serum levels of this antioxidant enzyme appeared more significantly increased than in OA patients with lower K/L grade (p < 0.001). The mtDNA haplogroups showed an influence on serum levels of catalase (p = 0.054), being carriers of the mtDNA haplogroup J those who showed higher serum levels than non-J carriers (p = 0.057). The increased levels of SOD2 in OA patients indicate an increased oxidative stress OA-related, therefore this antioxidant enzyme could be a suitable candidate biomarker for diagnosis of OA. Mitochondrial haplogroups significantly correlates with serum levels of catalase
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影响因子：
27.4
作者：
Rego-Perez, I.;Fernandez-Moreno, M.;Blanco, F. J.
通讯作者：
Blanco, F. J.
影响因子：
2.3
作者：
Andersson ML;Thorstensson CA;Roos EM;Petersson IF;Heinegård D;Saxne T
通讯作者：
Saxne T
影响因子：
--
作者：
Garnero, P;Ayral, X;Delmas, PD
通讯作者：
Delmas, PD
影响因子：
27.4
作者：
Rego, I.;Fernandez-Moreno, M.;Blanco, F. J.
通讯作者：
Blanco, F. J.
影响因子：
--
作者：
Aigner, Thomas;Fundel, Katrin;Bartnik, Eckart
通讯作者：
Bartnik, Eckart