Recovering coronavirus from large volumes of water.
Recovering coronavirus from large volumes of water.
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DOI：
10.1016/j.scitotenv.2020.143101
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发表时间：
2021-03-25
期刊：
影响因子：
--
通讯作者：
Sánchez G
中科院分区：
文献类型：
--
作者：
Cuevas-Ferrando E;Pérez-Cataluña A;Allende A;Guix S;Randazzo W;Sánchez G
对监测工具的需求以更好地控制正在进行的冠状病毒病（COVID-19）大流行是极其紧迫的，而排泄的病毒颗粒对水资源的污染提出了有待回答的令人担忧的问题。作为克服沿水循环监测SARS-CoV-2沿着技术局限性的第一步，我们评估了死端中空纤维超滤的分析性能，该超滤结合不同的二次浓缩选项，以浓缩来自大体积加标自来水、海水和地表水的病毒颗粒，以及两种定量RT-qPCR检测试剂盒。将SARS-CoV-2的包膜病毒替代物猪流行性腹泻病毒（PEDV）与孟戈病毒一起加标，我们证明PEG沉淀和SENS试剂盒从自来水中更好地回收了PEDV（13.10 ± 0.66%），而离心过滤是回收孟戈病毒的最佳选择，无论使用何种检测试剂盒。当比较高（10，000 ×g）和低（3500 ×g）离心速度对二级PEG基加标海水浓度的影响时，未发现统计学显著性差异，而通过NoInh试剂盒检测，观察到两种病毒均具有相当大的抑制作用。类似地，在用SENS-试剂盒或NoInh-试剂盒检测的表面沃茨中观察到PCR抑制剂和病毒颗粒的共浓缩，并且需要RNA稀释以该方法的总体灵敏度为代价来实现可接受的回收率。这些方法代表了调查SARS-CoV-2在不同水资源中发生的适当选择，并允许对大量水进行现场采样。
The need for monitoring tools to better control the ongoing coronavirus disease (COVID-19) pandemic is extremely urgent and the contamination of water resources by excreted viral particles poses alarming questions to be answered. As a first step to overcome technical limitations in monitoring SARS-CoV-2 along the water cycle, we assessed the analytical performance of a dead end hollow fiber ultrafiltration coupled to different options for secondary concentrations to concentrate viral particles from large volume of spiked tap water, seawater and surface water together with two quantitative RT-qPCR detection kits. Spiking the porcine epidemic diarrhea virus (PEDV), an enveloped virus surrogate for SARS-CoV-2, together with the mengovirus, we demonstrated that PEG-precipitation and SENS-kit better recovered PEDV (13.10 ± 0.66%) from tap water, while centrifugal filtration resulted the best option to recover mengovirus regardless of the detection kit used. No statistical significant differences were found when comparing high (10,000 ×g) and low (3500 ×g) centrifugation speeds for the secondary PEG- based concentration of spiked seawater, while considerable inhibition was observed for both viruses detected by NoInh-kit assay. Similarly, the co-concentration of PCR inhibitors and viral particles was observed in surface waters detected with either SENS-kit or NoInh-kit and RNA dilution was needed to achieve acceptable recoveries at the expenses of the overall sensitivity of the method. These methodologies represent suitable options to investigate SARS-CoV-2 occurrence in different water resources and allow to conduct on site sampling of large volume of water.
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DOI：
10.1016/j.coesh.2020.01.006
发表时间：
2020-08-01
影响因子：
--
作者：
Bofill-Mas, Silvia;Rusinol, Marta
通讯作者：
Rusinol, Marta
影响因子：
9.8
作者：
Heller, Leo;Mota, Cesar R.;Greco, Dirceu B.
通讯作者：
Greco, Dirceu B.
影响因子：
9.8
作者：
Guerrero-Latorre, Laura;Ballesteros, Isabel;Rios-Touma, Blanca
通讯作者：
Rios-Touma, Blanca
影响因子：
9.8
作者：
Haramoto, Eiji;Malla, Bikash;Kitajima, Masaaki
通讯作者：
Kitajima, Masaaki
影响因子：
9.8
作者：
Ahmed, Warish;Bertsch, Paul M.;Kitajima, Masaaki
通讯作者：
Kitajima, Masaaki