Physiological Basis and Transcriptional Profiling of Three Salt-Tolerant Mutant Lines of Rice.
Physiological Basis and Transcriptional Profiling of Three Salt-Tolerant Mutant Lines of Rice.
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大米的三种耐盐突变品系的生理基础和转录分析。
DOI：
10.3389/fpls.2016.01462
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发表时间：
2016
影响因子：
5.6
通讯作者：
Talón M
中科院分区：
文献类型：
--
作者：
Domingo C;Lalanne E;Catalá MM;Pla E;Reig-Valiente JL;Talón M
盐度是一个复杂的性状，影响包括水稻在内的许多作物的生长和生产力。突变诱导是培育耐盐植物的有效手段，它可以对具有相似遗传背景的植物进行分析，有助于了解植物的耐盐机制。本研究通过对盐敏感品种进行辐射诱变，获得了3个耐盐突变株系，并在高盐胁迫下对M2代植株进行了苗期筛选。这三个系，SaT 20，SaS 62，和SaT 58，表现出不同的反应，盐度，但表现出类似的表型野生型植物，除了SaT 20，显示较短的高度时，在盐的情况下生长。在盐条件下，所有三个突变体和亲本线表现出类似的产量下降，但在其他生理参数，如Na+积累在健康叶片的SaT 20，注册相关的差异。根中基因表达谱的微阵列分析揭示了突变体中常见和特异性反应的发生。这3个突变体均表现出响应基因表达上调、氧化还原过程激活和离子转运抑制。茉莉酸盐在植物响应盐的参与是显而易见的下调茉莉酸O-甲基转移酶的基因编码。处理脂质转运和代谢的基因，在一般情况下，上调除了在SaS 62，也表现出下调的基因参与离子转运和Ca 2+信号转导。两个最耐受的品种，SaS 62和SaT 20，表现出较低水平的转录参与K+吸收。生理研究和表达分析表明，三个系表现出不同的盐响应：SaT 20表现出高Na+含量的叶片，SaS 62表现出抑制脂质代谢和离子运输和SaT 58在这两个功能不同的盐响应。这些耐盐突变体的分析说明了这种特性的复杂性，证明了植物对盐度的反应的广度，包括同时合作的替代或互补机制。
Salinity is a complex trait that affects growth and productivity in many crops, including rice. Mutation induction, a useful tool to generate salt tolerant plants, enables the analysis of plants with similar genetic background, facilitating the understanding of the salt tolerance mechanisms. In this work, we generated three salt tolerant mutant lines by irradiation of a salt-sensitive cultivar plants and screened M2 plants at seedling stage in the presence of high salinity. These three lines, SaT20, SaS62, and SaT58, showed different responses to salinity, but exhibited similar phenotype to wild type plants, except SaT20 that displayed shorter height when grown in the absence of salt. Under salt conditions, all three mutants and the parental line showed similar reduction in yield, although relevant differences in other physiological parameters, such as Na+ accumulation in healthy leaves of SaT20, were registered. Microarray analyses of gene expression profiles in roots revealed the occurrence of common and specific responses in the mutants. The three mutants showed up-regulation of responsive genes, the activation of oxido-reduction process and the inhibition of ion transport. The participation of jasmonate in the plant response to salt was evident by down-regulation of a gene coding for a jasmonate O-methyltransferase. Genes dealing with lipid transport and metabolism were, in general, up-regulated except in SaS62, that also exhibited down-regulation of genes involved in ion transport and Ca2+ signal transduction. The two most tolerant varieties, SaS62 and SaT20, displayed lower levels of transcripts involved in K+ uptake. The physiological study and the description of the expression analysis evidenced that the three lines showed different responses to salt: SaT20 showed a high Na+ content in leaves, SaS62 presented an inhibition of lipid metabolism and ion transport and SaT58 differs in both features in the response to salinity. The analysis of these salt tolerant mutants illustrates the complexity of this trait evidencing the breadth of the plant responses to salinity including simultaneous cooperation of alternative or complementary mechanisms.
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