The liverwort Pellia endiviifolia shares microtranscriptomic traits that are common to green algae and land plants.
The liverwort Pellia endiviifolia shares microtranscriptomic traits that are common to green algae and land plants.
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DOI：
10.1111/nph.13220
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Szweykowska-Kulinska Z
中科院分区：
文献类型：
--
作者：
Alaba S;Piszczalka P;Pietrykowska H;Pacak AM;Sierocka I;Nuc PW;Singh K;Plewka P;Sulkowska A;Jarmolowski A;Karlowski WM;Szweykowska-Kulinska Z
苔类植物是现存陆地植物中最底层的类群。然而，对苔类的分子生物学知之甚少。基因表达已被研究，只有一个物种，地钱多形。特别是，没有报道来自苔类的microRNA（miRNA）序列。在这里，基于Illumina的下一代测序被用于鉴定小RNA，并分析Pellia endiviifolia的转录组和降解组。共鉴定了311个保守的植物miRNA家族，并发现了42个新的地钱特异性miRNA。RNA降解组分析表明，只有三种miRNAs（miR160，miR166和miR408）的靶mRNA在地钱和其他陆生植物之间是保守的。新的目标被确定为其余的保守的miRNA。此外，对degradome的分析允许鉴定13种新的地钱特异性miRNAs的靶标。有趣的是，其中三种苔类microRNA与先前报道的莱茵衣藻microRNA具有高度相似性。这是第一次观察到存在于代表性植物和地钱P. endiviifolia中的miRNA，但不存在于陆地植物中。对P. endivifolia微转录组的分析结果支持了先前研究的结论，即将地钱置于陆地植物进化树的根部。
Liverworts are the most basal group of extant land plants. Nonetheless, the molecular biology of liverworts is poorly understood. Gene expression has been studied in only one species, Marchantia polymorpha. In particular, no microRNA (miRNA) sequences from liverworts have been reported. Here, Illumina-based next-generation sequencing was employed to identify small RNAs, and analyze the transcriptome and the degradome of Pellia endiviifolia. Three hundred and eleven conserved miRNA plant families were identified, and 42 new liverwort-specific miRNAs were discovered. The RNA degradome analysis revealed that target mRNAs of only three miRNAs (miR160, miR166, and miR408) have been conserved between liverworts and other land plants. New targets were identified for the remaining conserved miRNAs. Moreover, the analysis of the degradome permitted the identification of targets for 13 novel liverwort-specific miRNAs. Interestingly, three of the liverwort microRNAs show high similarity to previously reported miRNAs from Chlamydomonas reinhardtii. This is the first observation of miRNAs that exist both in a representative alga and in the liverwort P. endiviifolia but are not present in land plants. The results of the analysis of the P. endivifolia microtranscriptome support the conclusions of previous studies that placed liverworts at the root of the land plant evolutionary tree of life.
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影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
14.9
作者：
Bielewicz D;Dolata J;Zielezinski A;Alaba S;Szarzynska B;Szczesniak MW;Jarmolowski A;Szweykowska-Kulinska Z;Karlowski WM
通讯作者：
Karlowski WM
影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S
影响因子：
5.8
作者：
Addo-Quaye, Charles;Miller, Webb;Axtell, Michael J.
通讯作者：
Axtell, Michael J.