Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients.
Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients.
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DOI：
10.1038/s41598-021-93857-7
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发表时间：
2021-07-09
影响因子：
4.6
通讯作者：
Ogretmen B
中科院分区：
文献类型：
--
作者：
Janneh AH;Kassir MF;Dwyer CJ;Chakraborty P;Pierce JS;Flume PA;Li H;Nadig SN;Mehrotra S;Ogretmen B
2019冠状病毒病大流行在世界各地造成医疗紧急情况。病情严重程度不均匀。目前还不清楚为什么一些SARS-CoV-2抗体阳性的人没有症状，而另一些人则表现出中度至重度的疾病症状。目前迫切需要可靠的生物标志物来早期发现这种疾病，以减少病毒的传播，并帮助做出早期治疗决定。生物活性鞘脂在调节病毒感染和与COVID-19严重程度相关的促炎反应方面发挥着至关重要的作用。然而，鞘脂在COVID-19发展或检测中的任何作用仍然未知。在这项研究中，血清鞘脂的脂质组学测量表明，与无症状患者相比，大多数（99.24%）SARS-CoV-2感染患者的鞘氨醇水平降低与症状性COVID-19的发展高度相关。大多数无症状个体（73%）的血清中酸性神经酰胺酶（AC）增加，通过Western印迹法测定，与SARS-CoV-2抗体阴性对照相比，鞘氨醇水平升高一致。AC蛋白在几乎所有有症状的患者血清中也都减少了，这与在纵向急性或恢复期COVID-19样本中测量的鞘氨醇水平降低有关。因此，鞘氨醇水平降低为早期识别无症状与有症状的COVID-19患者提供了敏感和选择性的血清学生物标志物。
COVID-19 pandemic exerts a health care emergency around the world. The illness severity is heterogeneous. It is mostly unknown why some individuals who are positive for SARS-CoV-2 antibodies stay asymptomatic while others show moderate to severe disease symptoms. Reliable biomarkers for early detection of the disease are urgently needed to attenuate the virus’s spread and help make early treatment decisions. Bioactive sphingolipids play a crucial role in the regulation of viral infections and pro-inflammatory responses involved in the severity of COVID-19. However, any roles of sphingolipids in COVID-19 development or detection remain unknown. In this study, lipidomics measurement of serum sphingolipids demonstrated that reduced sphingosine levels are highly associated with the development of symptomatic COVID-19 in the majority (99.24%) SARS-CoV-2-infected patients compared to asymptomatic counterparts. The majority of asymptomatic individuals (73%) exhibited increased acid ceramidase (AC) in their serum, measured by Western blotting, consistent with elevated sphingosine levels compared to SARS-CoV-2 antibody negative controls. AC protein was also reduced in almost all of the symptomatic patients’ serum, linked to reduced sphingosine levels, measured in longitudinal acute or convalescent COVID-19 samples. Thus, reduced sphingosine levels provide a sensitive and selective serologic biomarker for the early identification of asymptomatic versus symptomatic COVID-19 patients.
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影响因子：
30.5
作者：
Chang CH;Pearce EL
通讯作者：
Pearce EL
DOI：
10.33594/000000194
发表时间：
2019-01-01
期刊：
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
影响因子：
--
作者：
Carstens, Henning;Schumacher, Fabian;Gulbins, Erich
通讯作者：
Gulbins, Erich
影响因子：
29
作者：
Chatterjee S;Daenthanasanmak A;Chakraborty P;Wyatt MW;Dhar P;Selvam SP;Fu J;Zhang J;Nguyen H;Kang I;Toth K;Al-Homrani M;Husain M;Beeson G;Ball L;Helke K;Husain S;Garrett-Mayer E;Hardiman G;Mehrotra M;Nishimura MI;Beeson CC;Bupp MG;Wu J;Ogretmen B;Paulos CM;Rathmell J;Yu XZ;Mehrotra S
通讯作者：
Mehrotra S
影响因子：
64.5
作者：
Karki R;Sharma BR;Tuladhar S;Williams EP;Zalduondo L;Samir P;Zheng M;Sundaram B;Banoth B;Malireddi RKS;Schreiner P;Neale G;Vogel P;Webby R;Jonsson CB;Kanneganti TD
通讯作者：
Kanneganti TD
影响因子：
15.9
作者：
Chen, Guang;Wu, Di;Ning, Qin
通讯作者：
Ning, Qin