Use of the light-dark box to compare the anxiety-related behavior of virgin and postpartum female rats.
Use of the light-dark box to compare the anxiety-related behavior of virgin and postpartum female rats.
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DOI：
10.1016/j.pbb.2011.08.002
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发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
Lonstein JS
中科院分区：
文献类型：
--
作者：
Miller SM;Piasecki CC;Lonstein JS
产后雌性啮齿动物比哺乳期的处女更不焦虑，这种差异有助于母鼠充分照顾幼崽并保卫巢穴。在许多行为模式中都观察到了产后低焦虑，但之前使用明暗盒子的研究结果并不一致。在这里，我们重新检查了明暗盒子的有用性，以评估产后和哺乳期处女大鼠在焦虑相关行为方面的差异。我们发现了生殖状态的显著影响，比如，母狗在光室里呆的时间比哺乳期处女多。这种差异需要最近与幼崽的身体接触，因为与幼崽分开四个小时可以将水坝在光室中花费的时间减少一半，这与我们之前在高架加号迷宫中测试的垃圾分离水坝的情况类似。最后，我们检查了DAMS在明暗盒子中的低焦虑行为是否依赖于高GABAA受体活性，方法是使用(+)-荷包牡丹碱靶向GABA位点，FG-7142靶向苯二氮卓类位点，并使用戊四氮靶向印迹毒素位点，从而抑制不同结合位点的受体。只有戊四唑在水坝中一直会引起焦虑，而对哺乳期的处女几乎没有影响。因此，光-暗盒可以成为研究产后和发情处女大鼠焦虑相关行为差异的有用范例，这种差异受到母鼠最近与幼鼠接触的影响，以及GABAA受体神经传递尤其受印防己毒素部位活动影响。
Postpartum female rodents are less anxious than diestrous virgins and this difference contributes to dams’ ability to adequately care for pups and defend the nest. Low postpartum anxiety has been observed in many behavioral paradigms but the results of previous studies using the light-dark box have been inconsistent. We here reexamined the usefulness of the light-dark box to assess differences between postpartum and diestrous virgin female rats in anxiety-related behavior. We found a significant effect of reproductive state, such that dams spent more time in the light chamber than did diestrous virgins. This difference required recent physical contact with pups because a four-hour separation from pups reduced dams’ time spent in the light chamber by half, similar to what we previously found for litter-separated dams tested in an elevated plus maze. Lastly, we examined if dams’ low-anxiety behavior in the light-dark box depends on high GABAA receptor activity by inhibiting the receptor at different binding sites using (+)-Bicuculline to target the GABA site, FG-7142 to target the benzodiazepine site, and pentylenetetrazol to target the picrotoxin site. Only pentylenetetrazol was consistently anxiogenic in dams, while having little effect in diestrous virgins. Thus, the light-dark box can be a useful paradigm to study differences between postpartum and diestrous virgin female rats in their anxiety-related behaviors, and this difference is influenced by dams’ recent contact with pups and GABAA receptor neurotransmission particularly affected by activity at the picrotoxin site.
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影响因子：
3.4
作者：
Dalvi, A;Rodgers, RJ
通讯作者：
Rodgers, RJ
影响因子：
3.5
作者：
Agrati, D.;Zuluaga, M. J.;Ferreira, A.
通讯作者：
Ferreira, A.
影响因子：
4.7
作者：
FILE, SE;LISTER, RG
通讯作者：
LISTER, RG
DOI：
10.1085/jgp.200609639
发表时间：
2007-02
期刊：
The Journal of general physiology
影响因子：
--
作者：
Bali M;Akabas MH
通讯作者：
Akabas MH
影响因子：
3.4
作者：
COLE, BJ;HILLMANN, M;JONES, GH
通讯作者：
JONES, GH