Intermediate rate atomic trajectories of RNA by solid-state NMR spectroscopy.
Intermediate rate atomic trajectories of RNA by solid-state NMR spectroscopy.
复制标题
DOI：
10.1021/ja907515s
复制
发表时间：
2010-01-13
影响因子：
15
通讯作者：
Varani, Gabriele
中科院分区：
文献类型：
--
作者：
Olsen, Greg L.;Bardaro, Michael F., Jr.;Echodu, Dorothy C.;Drobny, Gary P.;Varani, Gabriele
Many RNAs undergo large conformational changes in response to the binding of proteins and small molecules. However, when RNA functional dynamics occur in the ns-μs time scale they become invisible to traditional solution NMR relaxation methods. Residual dipolar couplings methods have revealed the presence of extensive ns-μs domain motions in HIV-1 TAR RNA, but this technique lacks information on the rates of motions. We have used solid-state deuterium NMR to quantitatively describe trajectories of key residues in TAR by exploiting the sensitivity of this technique to motions that occur in the ns-μs regime. Deuterium lineshape and relaxation data were used to model motions of residues within the TAR binding interface. The resulting motional models indicate that two functionally essential bases within the single stranded bulge sample both the free and Tat-bound conformations on the microsecond timescale in the complete absence of the protein. Thus, our results strongly support a conformational capture mechanism for recognition: the protein does not induce a new RNA structure, but instead captures an already-populated conformation.
登录
查看更多内容
影响因子：
2.9
作者：
Leulliot, N;Varani, G
通讯作者：
Varani, G
影响因子：
14.9
作者：
Bardaro, Michael F., Jr.;Shajani, Zahra;Varani, Gabriele
通讯作者：
Varani, Gabriele
影响因子：
56.9
作者：
Boehr, David D.;McElheny, Dan;Wright, Peter E.
通讯作者：
Wright, Peter E.
影响因子：
2.2
作者：
Schurr, JM;Fujimoto, BS;Robinson, BH
通讯作者：
Robinson, BH
影响因子：
14.9
作者：
Olsen GL;Edwards TE;Deka P;Varani G;Sigurdsson ST;Drobny GP
通讯作者：
Drobny GP