Isolation of extracellular vesicles: Determining the correct approach (Review).
Isolation of extracellular vesicles: Determining the correct approach (Review).
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DOI：
10.3892/ijmm.2015.2194
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发表时间：
2015-07
影响因子：
5.4
通讯作者：
Baj-Krzyworzeka M
中科院分区：
文献类型：
--
作者：
Szatanek R;Baran J;Siedlar M;Baj-Krzyworzeka M
细胞外囊泡（EVs）的发现修订了对细胞间通讯的解释。现在已经确定ev在凝血、炎症、癌症和干细胞更新和扩增中发挥重要作用。它们的释放呈现出一个有趣的、运输/贩运生物活性分子的网络，这些分子能够以各种方式到达并调节靶细胞的功能/行为。此外，ev在各种体液中的存在表明它们有潜力作为生物标志物和预后指标，用于监测/监测各种疾病。尽管多年来积累了大量关于电动汽车的知识，但仍然存在与隔离电动汽车的正确方法相关的基本问题。这篇综述包括目前可用的EV隔离技术的知识。这篇综述的目的是让经验丰富的研究人员和新手都意识到，不同类型的电动汽车需要独特的隔离方法。实现这种“独特性”是对电动汽车进行全面评估的正确方向的第一步。
The discovery of extracellular vesicles (EVs) has revised the interpretation of intercellular communication. It is now well established that EVs play a significant role in coagulation, inflammation, cancer and stem cell renewal and expansion. Their release presents an intriguing, transporting/trafficking network of biologically active molecules, which are able to reach and modulate the function/behavior of the target cells in a variety of ways. Moreover, the presence of EVs in various body fluids points to their potential for use as biomarkers and prognostic indicators in the surveillance/monitoring of a variety of diseases. Although vast knowledge on the subject of EVs has accumulated over the years, there are still fundamental issues associated with the correct approach for their isolation. This review comprises the knowledge on EV isolation techniques that are currently available. The aim of this review was to make both experienced researchers and newcomers to the field aware that different types of EVs require unique isolation approaches. The realization of this ‘uniqueness’ is the first step in the right direction for the complete assessment of EVs.
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