Genetic analysis of the transcriptional arrangement of Azotobacter vinelandii alginate biosynthetic genes: identification of two independent promoters
Genetic analysis of the transcriptional arrangement of Azotobacter vinelandii alginate biosynthetic genes: identification of two independent promoters
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维氏固氮菌藻酸盐生物合成基因转录排列的遗传分析：两个独立启动子的鉴定
DOI：
10.1111/j.1365-2958.1996.tb02554.x
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发表时间：
1996
影响因子：
3.6
通讯作者：
G. Soberón
中科院分区：
文献类型：
--
作者：
L. Lloret;Rita Barreto;Renato León;S. Moreno;J. Martínez;G. Espín;G. Soberón
对棕色固氮菌和铜绿假单胞菌产生的藻酸盐生物合成的研究可能会导致不同的生物技术应用。本文报道了A. vinelandii algA，编码双功能酶磷酸甘露糖异构酶-鸟苷二磷酸-D-甘露糖焦磷酸化酶（PMI-GMP）的基因。该基因是通过对野油菜黄单胞菌黄原胶生产菌株的互补作用而筛选出来的。campestris xanB突变体，其缺乏这种酶活性。所选择的互补粘粒克隆，除了含有algA之外，还存在编码藻酸裂解酶活性的基因（algL），并且其中一些还含有编码GDP-甘露糖脱氢酶的algD。我们在这里提出的A. vinelandii染色体区域，包括algD及其启动子区域，algA和algL，表明，如以前报道的铜绿假单胞菌，A. vinelandii具有一簇生物合成藻酸盐基因。我们提供的证据表明，在该区域存在一个algD非依赖性启动子，它至少转录algL和algA，并且以不同于algD启动子的方式进行调控。
The study of alginate biosynthesis, the exopolysac charide produced by Azotobacter vinelandii and Pseudomonas aeruginosa, might lead to different bio‐technological applications. Here we report the cloning of A. vinelandii algA, the gene coding for the bifunctional enzyme phosphomannose isomerase‐guano‐sine diphospho‐D‐mannose pyrophosphorylase (PMI‐GMP). This gene was selected by the complementation for xanthan gum production of Xanthomonas campestris pv. campestris xanB mutants, which lack this enzymatic activity. The complementing cosmid clones selected, besides containing algA, presented a gene coding for an alginate lyase activity (algL), and some of them also contained algD which codes for GDP‐mannose dehydrogenase. We present here the characterization of the A. vinelandii chromosomal region comprising algD and its promoter region, algA and algL, showing that, as previously reported for P. aeruginosa, A. vinelandii has a cluster of the biosynthetic alginate genes. We provide evidence for the presence of an algD‐independent promoter in this region which transcribes at least algL and algA, and which is regulated in a manner that differs from that of the algD promoter.
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影响因子：
3.5
作者：
DARZINS, A;FRANTZ, B;CHAKRABARTY, AM
通讯作者：
CHAKRABARTY, AM
影响因子：
15.9
作者：
T. B. May;A. Chakrabarty
通讯作者：
T. B. May;A. Chakrabarty
影响因子：
3.5
作者：
BOYD, A;GHOSH, M;CHAKRABARTY, AM
通讯作者：
CHAKRABARTY, AM
影响因子：
3.2
作者：
Duevel, Juliane;Bense, Sarina;Haeussler, Susanne
通讯作者：
Haeussler, Susanne
DOI：
10.1073/pnas.92.17.7941
发表时间：
1995-08-15
影响因子：
11.1
作者：
HERSHBERGER, CD;YE, RW;CHAKRABARTY, AM
通讯作者：
CHAKRABARTY, AM