Salivary immunoglobulin G assay to diagnose Helicobacter pylori infection in children
Salivary immunoglobulin G assay to diagnose Helicobacter pylori infection in children
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唾液免疫球蛋白G检测诊断儿童幽门螺杆菌感染
DOI：
10.1128/jcm.35.12.3358-3360.1997
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发表时间：
1997
影响因子：
9.4
通讯作者：
Francesco Pallone
中科院分区：
文献类型：
--
作者：
Francesco Luzza;G. Oderda;Maria Maletta;M. Imeneo;L. Mesuraca;Elena Chioboli;Piero Lerro;Stefano Guandalini;Francesco Pallone
通过对112例诊断性胃镜检查患儿的胃活检标本进行组织病理学（Giemsa染色）和生化（尿素酶快速试验）检查，评价了用于测量唾液中幽门螺杆菌特异性免疫球蛋白G（IgG）和伊加的内部酶联免疫吸附试验（ELISA）。血清H.用同样的ELISA法检测了50名儿童的幽门螺杆菌IgG。唾液H. pylori IgG水平在H. pylori阳性（n = 57），H. pylori阴性55例（P < 0.001）。唾液IgG检测的敏感性和特异性分别为93%和82%，阳性和阴性预测值分别为84%和92%，准确性为87.5%。唾液H. pylori伊加不能区分H. pylori阳性的H.幽门阴性的孩子血清H. pylori IgG阳性率略高于唾液H. pylori IgG。唾液IgG试验可作为H.儿童幽门螺杆菌感染
An in-house enzyme-linked immunosorbent assay (ELISA) for measurement of Helicobacter pylori-specific immunoglobulin G (IgG) and IgA in saliva was evaluated by comparison with histopathologic (Giemsa staining) and biochemical (urease quick test) examination of gastric biopsy specimens obtained from 112 children referred for diagnostic gastroscopy. Serum H. pylori IgG was also measured in a subgroup of 50 children by the same ELISA. Salivary H. pylori IgG levels were significantly higher in H. pylori-positive (n = 57) than in H. pylori-negative (n = 55) children (P < 0.001). The sensitivity and specificity of the salivary IgG test were 93 and 82%, respectively; the positive and negative predictive values were 84 and 92%, respectively; and the accuracy was 87.5%. Salivary H. pylori IgA did not distinguish H. pylori-positive from H. pylori-negative children. The performance of serum H. pylori IgG was slightly (3 to 6%) better than that of salivary H. pylori IgG. The salivary IgG test can be considered a useful tool for the screening of H. pylori infection in children.
胃十二指肠疾病儿童幽门弯曲杆菌外膜蛋白血清 IgG 抗体。
DOI：
10.1093/infdis/159.3.586
发表时间：
1989
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Czinn,S;Carr,H;Sheffler,L;Aronoff,S
通讯作者：
Aronoff,S
影响因子：
158.5
作者：
PARSONNET, J;HANSEN, S;FRIEDMAN, GD
通讯作者：
FRIEDMAN, GD