Enhanced metabolite profiling using a redesigned atmospheric pressure chemical ionization source for gas chromatography coupled to high-resolution time-of-flight mass spectrometry
Enhanced metabolite profiling using a redesigned atmospheric pressure chemical ionization source for gas chromatography coupled to high-resolution time-of-flight mass spectrometry
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使用重新设计的气相色谱大气压化学电离源与高分辨率飞行时间质谱联用增强代谢物分析
DOI：
10.1007/s00216-015-8824-x
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发表时间：
2015
影响因子：
4.3
通讯作者：
Dettmer K
中科院分区：
文献类型：
--
作者：
Wachsmuth CJ;Hahn TA;Oefner PJ;Dettmer K
一种改进的大气压化学电离（APCI II）源气相色谱-高分辨率飞行时间质谱（GC-HRTOFMS）进行了比较，其第一代前身的脂肪酸甲酯，代谢物标准品的甲氧基-三甲基甲硅烷基衍生物，和细胞培养上清液的分析。气体湍流和化学背景的减少以及APCI II源的优化加热导致了更窄的峰和更高的重复性，特别是对于后期洗脱的化合物。此外，对于研究的所有代谢物标准品，APCI II使定量下限的中位值降低超过4倍，降至0.002-3.91 μM沿着，线性范围平均增加20%，达到近3个数量级，R2值高于0.99。这使得GC-APCI-HRTOFMS的整体性能与全面的二维气相色谱（GC × GC）-电子电离（EI）-TOFMS的整体性能相当。最后，在从APCI I源切换到APCI II源时，可以从胰腺癌细胞上清液的代谢物指纹中提取的信噪比大于20的峰的数量增加了一倍以上。同时，鉴别的代谢物数量从36种增加到48种。总之，改进的APCI II源使GC-APCI-HRTOFMS成为代谢组学和其他领域基于EI的GC-MS技术的一个很好的替代方案。
An improved atmospheric pressure chemical ionization (APCI II) source for gas chromatography–high-resolution time-of-flight mass spectrometry (GC–HRTOFMS) was compared to its first-generation predecessor for the analysis of fatty acid methyl esters, methoxime-trimethylsilyl derivatives of metabolite standards, and cell culture supernatants. Reductions in gas turbulences and chemical background as well as optimized heating of the APCI II source resulted in narrower peaks and higher repeatability in particular for late-eluting compounds. Further, APCI II yielded a more than fourfold median decrease in lower limits of quantification to 0.002–3.91 μM along with an average 20 % increase in linear range to almost three orders of magnitude withR2values above 0.99 for all metabolite standards investigated. This renders the overall performance of GC–APCI–HRTOFMS comparable to that of comprehensive two-dimensional gas chromatography (GC × GC)–electron ionization (EI)–TOFMS. Finally, the number of peaks with signal-to-noise ratios greater than 20 that could be extracted from metabolite fingerprints of pancreatic cancer cell supernatants upon switching from the APCI I to the APCI II source was more than doubled. Concomitantly, the number of identified metabolites increased from 36 to 48. In conclusion, the improved APCI II source makes GC–APCI–HRTOFMS a great alternative to EI-based GC–MS techniques in metabolomics and other fields.
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影响因子：
4.1
作者：
Almstetter, Martin F.;Appel, Inka J.;Oefner, Peter J.
通讯作者：
Oefner, Peter J.
影响因子：
7.4
作者：
Christian J. Wachsmuth;K. Dettmer;S. Lang;M. Mycielska;P. Oefner
通讯作者：
Christian J. Wachsmuth;K. Dettmer;S. Lang;M. Mycielska;P. Oefner
影响因子：
2
作者：
Bristow, Tony;Harrison, Mark;Sims, Martin
通讯作者：
Sims, Martin
使用液体点电极的毛细管大气压化学电离。
DOI：
10.1002/rcm.6944
发表时间：
2014
期刊：
Rapid communications in mass spectrometry : RCM
影响因子：
--
作者：
Sonja Klee;Marco Thinius;K. Brockmann;T. Benter
通讯作者：
T. Benter
影响因子：
4.3
作者：
Schiewek, Ralf;Lorenz, Matthias;Schmitz, Oliver J.
通讯作者：
Schmitz, Oliver J.